Kumar, S., et al. · 2016 · Plant Cell research
doi:10.1105/tpc.16.00062 PMID:27650333 PMC5134972
| Gene ID | Name | Evidence / Role | Function (this paper) |
|---|---|---|---|
| — | MpDTPS1 missing | experimental subject | Diterpene synthase functionally characterized as an ent-atiseranol synthase; major product atiseran-16-ol confirmed by NMR, with minor ent-kaurene and ent-atiserene. |
| — | MpDTPS3 missing | experimental subject | Class II diterpene cyclase functionally characterized as an ent-copalyl diphosphate synthase; sole product copalol (dephosphorylated CPP); stereochemistry confirmed by coexpression with kaurene synthases. |
| — | MpDTPS4 missing | experimental subject | Class I diterpene synthase functionally characterized as an ent-kaurene synthase; produces ent-kaurene when coexpressed with ent-CPP-producing synthases. |
| Mp6g04320 | MpFTPSL2 | experimental subject | Sesquiterpene synthase generating 22 in vitro products from FPP (49.54 pmol/h/ug), of which only five were detected in planta. |
| Mp3g19720 | MpFTPSL8 | experimental subject | Most versatile enzyme of the set, accepting both mono- and sesquiterpene substrates; slightly more active with NPP than GPP and yields cis-beta-ocimene. mRNA 5-10 fold more abundant than other TPS-like contigs. |
| Mp4g10600 | MpMTPSL1 | experimental subject | Monoterpene/sesquiterpene synthase-like gene functionally characterized by enzyme assay; showed modest activity with all-trans FPP (2.41 pmol/h/ug). Member of the noncanonical TPS-like family lacking the DDXXD motif. |
| Mp3g18500 | MpMTPSL2 | experimental subject | Functionally characterized as a limonene synthase with unusual preference for neryl diphosphate (NPP) over GPP (~25-fold; 108.1 pmol/h/ug). Expression higher in early development, correlating with limonene accumulation. |
| Mp4g04780 | MpMTPSL3 | experimental subject | Sesquiterpene synthase showing the highest specific activity among the set with all-trans FPP (525.17 pmol/h/ug); produces multiple sesquiterpenes including (-)-alpha-gurjunene. |
| Mp5g21360 | MpMTPSL4 | experimental subject | Sesquiterpene synthase preferring all-trans FPP (46.59 pmol/h/ug); generates (-)-alpha-gurjunene, 5-hydroxy-alpha-gurjunene and related sesquiterpene alcohols. Product structures extensively characterized; mRNA increases over development. |
| Mp4g05900 | MpMTPSL5 | experimental subject | Sesquiterpene synthase-like gene functionally characterized; preferred substrate all-trans FPP (56.23 pmol/h/ug). |
| Mp4g05010 | MpMTPSL8 | experimental subject | Monoterpene synthase-like gene with modest activity using GPP (1.04 pmol/h/ug). |
| Mp1g25500 | MpMTPSL9 | experimental subject | Sesquiterpene synthase with high activity on all-trans FPP (250.5 pmol/h/ug), the second-highest specific activity among the characterized synthases. |
| — | MpCPS missing | experimental comparator | Refers to the ent-copalyl diphosphate synthase function; in this paper realized by MpDTPS3, discussed as a separate monofunctional enzyme orthologous to vascular-plant CPS. |
| — | MpKS missing | experimental comparator | Refers to the ent-kaurene synthase function; in this paper realized by MpDTPS4, discussed as a separate monofunctional enzyme orthologous to vascular-plant KS. |
| — | MpDTPS2 missing | sequence background | Putative diterpene synthase identified by sequence; not functionally characterized due to failed full-length cDNA recovery and a frame-shift mutation in the coding sequence. |