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MpWIP regulates air pore complex development in the liverwort Marchantia polymorpha.

Jones, V. A. S., et al. · 2017 · Development   research

doi:10.1242/dev.144287   PMID:28174248   PMC5399667

Linked genes (4)  1 core 3 peripheral

Gene ID Name Evidence / Role Function (this paper)
Mp1g09500 MpWIP experimental subject Primary subject: identified via T-DNA mutant vj7 (insertion upstream, ~4x higher transcript), overexpression (proOsACT:MpWIP) and amiRNA knockdown, plus SRDX/VP16 dominant constructs and proMpWIP:YFP-NLS reporter. Overexpression/repression caused ectopic dorsal rhizoids; knockdown gave defective air pores. Concluded MpWIP is necessary for air pore complex morphogenesis.
MpACT missing experimental tool Used as a reference gene for qPCR normalization.
Mp3g25140 MpAPT experimental tool Used as a reference gene for qPCR normalization.
Mp1g26670 MpMIR160 experimental tool Used as the backbone scaffold for building artificial microRNAs (amiR-MpWIP) targeting the MpWIP 3' UTR and CDS.