Carella, P., et al. · 2018 · Proceedings of the National Academy of Sciences USA: doi/10.1073/pnas.1717900115 research
doi:10.1073/pnas.1717900115 PMID:29615512 PMC5910834
| Gene ID | Name | Evidence / Role | Function (this paper) |
|---|---|---|---|
| Mp5g16920 | MpDIR33 | experimental subject | Dirigent-like defense marker; expression was significantly higher in infected plants than controls by qRT-PCR, consistent with prior reports of up-regulation in pathogen-treated moss. |
| MpVg00760 | MpRAB11A paper
MpRAB11AY nomenclature |
experimental subject | Endosomal Rab GTPase; overexpressed mCitrine-MpRab11A accumulated at pathogen interfaces and intracellular hyphae during infection, relocalizing from endosomes, indicating trafficking recruitment to infection structures. |
| Mp8g13820 | MpRAB7 | experimental subject | Endosomal Rab GTPase; mCitrine-MpRab7 transgenic plants showed strong labeling of intracellular infection structures and hyphae in living Marchantia cells at 3 dpi, indicating recruitment of trafficking machinery to the pathogen interface. |
| Mp2g19600 | MpSYP13B | experimental subject | Membrane syntaxin significantly up-regulated from 2 to 4 dpi during Phytophthora palmivora infection. mCitrine-MpSYP13B strongly labeled intracellular infection structures, showing focal accumulation proximal to hyphal buds and complete labeling around intracellular hyphae, suggesting it evolved to mark intracellular accommodation structures in liverworts. |
| Mp5g21510 | MpPR9l | experimental comparator | Peroxidase used as a host defense marker; transcript levels were significantly higher in P. palmivora-colonized TAK1 plants than mock controls by 3 dpi, and MpPRX:GUS reporter lines showed strong GUS induction in the photosynthetic layer during infection. |
| Mp7g13190 | MpSYP13A | experimental comparator | Paralogous syntaxin used as contrast to MpSYP13B; its expression was not affected during colonization and its localization was unchanged by intracellular infection structures. |
| — | MpACT missing | sequence tool | Actin gene used as an internal reference for qRT-PCR normalization and as a M. polymorpha biomass marker for relative pathogen biomass quantification. |
| Mp3g23400 | MpEF1 | sequence tool | Elongation factor 1-alpha used as an internal qRT-PCR reference and host biomass marker against which P. palmivora PpEF1a biomass was quantified. |