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A cis-acting bidirectional transcription switch controls sexual dimorphism in the liverwort.

Hisanaga, T., et al. · 2019a · The EMBO Journal   research

doi:10.15252/embj.2018100240   PMID:30609993   PMC6418429

Linked genes (5)  2 core 3 peripheral

Gene ID Name Evidence / Role Function (this paper)
Mp1g17210 MpFGMYB experimental subject Key regulator of female sexual differentiation; loss-of-function caused female-to-male sex conversion and it is female-specifically expressed. Suppressed in cis by the antisense gene SUF at the same locus, making it the central subject of the sexual-dimorphism switch.
Mp1g17205 MpSUF missing experimental subject cis-acting antisense gene at the MpFGMYB locus; suf loss-of-function mutations (1-kb TSS-region deletion) caused male-to-female conversion via male-specific antisense lncRNA accumulation. SUF-mediated MpFGMYB suppression is locus-specific and not rescued by SUF overexpression.
Mp3g23400 MpEF1 experimental tool Constitutively expressed gene used as the internal control for RT-PCR normalization.
Mp6g01560 MpLC7 experimental background DYNEIN LIGHT CHAIN7 male/sperm-morphogenesis marker expressed in antheridiophores, used alongside MpRPM to confirm the male program in Mpfgmyb mutants.
MpRPM missing experimental background PROTAMINE-LIKE male/sperm-morphogenesis marker shown by expression analysis to be ectopically expressed in antheridiophores of Mpfgmyb mutants.