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Genome-wide analysis of MpBHLH12, a IIIf basic helix-loop-helix transcription factor of Marchantia polymorpha.

Arai, H., et al. · 2019 · Journal of Plant Research   research

doi:10.1007/s10265-019-01095-w   PMID:30840209   PMC7196945

Linked genes (6)  1 core 5 peripheral

Gene ID Name Evidence / Role Function (this paper)
Mp2g05070 MpBHLH12 experimental subject Main subject: a IIIf bHLH transcription factor of M. polymorpha analyzed by overexpression and RNA-Seq. Phylogeny places it in the IIIf clade; ox lines showed reduced gemma cup size and number. Reporter assays indicate it acts as a repressor rather than activator, with no anthocyanin accumulation despite MpMYB14 upregulation.
Mp5g19050 MpR2R3-MYB14 experimental comparator Anthocyanin/phenylpropanoid regulator; significantly up-regulated in MpBHLH12ox lines and compared as a parallel GRN, though its and MpBHLH12's networks function distinctly.
Mp6g13500
UGT paper
MpUGT25 nomenclature
sequence comparator UDP-glucosyl transferase (Mapoly0047s0001) marker, the only gene down-regulated in MpBHLH12ox but up-regulated in MpMYB14 lines, marking network divergence.
Mp3g23400 MpEF1 experimental tool Elongation factor-1a (Mapoly0024s0116) used as the reference gene for qRT-PCR normalization.
Mp2g22700 MpPKS21 sequence tool Chalcone synthase (Mapoly0072s0061) flavonoid-pathway marker, significantly up-regulated in MpBHLH12ox lines in RNA-Seq comparison.
Mp3g07510 MpMYB02 citation background Reported phenolic (marchantin) biosynthesis regulator; showed no significant expression difference between transgenic and wild-type lines.