Gutsche, N., et al. · 2017 · Plant Direct research
doi:10.1002/pld3.30 PMID:31245678 PMC6508501
| Gene ID | Name | Evidence / Role | Function (this paper) |
|---|---|---|---|
| Mp5g10600 | MpROXY1 | experimental subject | Functionally studied ROXY glutaredoxin: complemented Arabidopsis roxy1-2 flower defect, interacted with MpTGA/PAN by Y2H, BiFC and FRET-FLIM (C-terminal deletion MpROXY1Δ14 abolished binding), and gave redox-dependent supershift on the as-1-like motif by EMSA. Overexpression in M. polymorpha caused severe growth defects requiring active-site cysteines, and GFP fusions colocalized with active RNAPII; shown conserved with Arabidopsis ROXY1. |
| Mp6g13210 | MpROXY2 | experimental subject | Functionally studied ROXY glutaredoxin: partially complemented Arabidopsis roxy1-2 petals and interacted (more weakly than MpROXY1) with MpTGA/PAN by Y2H, BiFC and FRET-FLIM, giving an incomplete redox-dependent supershift on the as-1-like motif by EMSA. Overexpression caused cysteine-dependent growth defects and in situ mRNA overlapped MpTGA in thallus tips and gemmae. |
| Mp2g13330 | MpTGA | experimental subject | Functionally studied TGA transcription factor: bound the as-1-like motif specifically by EMSA with redox sensitivity mediated jointly by cysteines C143/C231 (oxidation reduced binding, reversed by DTT), and formed redox-dependent supershifted complexes with MpROXY1/2. Interacted with ROXYs by Y2H/BiFC/FRET-FLIM and expressed in meristematic zones and gemmae overlapping MpROXY1/2. |
| Mp3g23400 | MpEF1 | sequence tool | Its promoter (MpEF1alpha) was used to drive ectopic MpROXY1/MpROXY2 CDS expression in M. polymorpha overexpression lines. |