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An Evolutionarily Conserved Coreceptor Gene Is Essential for CLAVATA Signaling in Marchantia polymorpha.

Takahashi, G., et al. · 2021 · Frontiers in Plant Science   research

doi:10.3389/fpls.2021.657548   PMID:33927741   PMC8076897

Linked genes (7)  1 core 6 peripheral

Gene ID Name Evidence / Role Function (this paper)
Mp7g14210 MpCIK1 experimental subject Single CIK ortholog (Mp7g14210); CRISPR knockouts (Mpcik-ge) are insensitive to MpCLE2 peptide and phenocopy Mpclv1. Co-IP shows MpCIK weakly associates with both MpCLV1 and MpTDR, acting as a coreceptor for CLE perception. Partly involved in MpCLE1/TDIF signaling.
Mp1g27720 MpCLV1 experimental comparator CLV3 receptor; knockouts (Mpclv1-ge) form narrow apical meristems insensitive to MpCLE2 and suppress overexpression phenotypes.
Mp1g09960 MpTDR experimental comparator TDIF receptor that weakly associates with MpCIK in co-IP, implicated in MpCLE1 signaling.
Mp6g07050 MpCLE1 experimental tool TDIF-type CLE homolog (Mp6g07050); proMpYUC2:MpCLE1 overexpression gives small twisted thalli, with only partial MpCIK involvement.
Mp5g18050 MpCLE2 experimental tool CLV3-type CLE homolog (Mp5g18050); synthetic peptide expands apical notches in WT but not in Mpcik/Mpclv1, and overexpression reduces gemma cups.
Mp8g08780 MpYUC2 experimental tool Used as a promoter (proMpYUC2:GUS) marking the stem cell zone, which is reduced in Mpcik and Mpclv1 mutants.
Mp7g09160 MpSERK sequence background One of three subclass-II LRR-RLK genes in M. polymorpha (Mp7g09160), grouped phylogenetically with Arabidopsis SERK genes; not functionally analyzed here.