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Microtubule associated protein WAVE DAMPENED2-LIKE (WDL) controls microtubule bundling and the stability of the site of tipgrowth in Marchantia polymorpha rhizoids.

Champion, C., et al. · 2021 · PLoS Genetics   research

doi:10.1371/journal.pgen.1009533   PMID:34086675   PMC8177534

Linked genes (6)  3 core 3 peripheral

Gene ID Name Evidence / Role Function (this paper)
missing experimental subject Locus encoding MpNEK (NIMA-RELATED PROTEIN KINASE), the causative gene for the wavy-rhizoid phenotype identified by genome sequencing of UV mutants.
Mp5g04500 MpNEK experimental subject NIMA-RELATED kinase that destabilizes microtubules at growing tips; loss-of-function mutants produce wavy rhizoids.
Mp7g06100 MpWDL experimental subject WAVE DAMPENED2-LIKE microtubule-binding protein, central subject of the paper, promoting microtubule bundling and longitudinal organization along the rhizoid shank.
Mp3g23400 MpEF1 experimental tool Reference gene used to normalize transcript levels in qPCR expression analyses of MpWDL and related targets.
MpRabA4 missing experimental tool Rab GTPase used as a YFP-tagged endomembrane/secretory-vesicle marker to track trafficking toward the apical dome during tip growth.
MpTUB1 experimental tool Tubulin used as a GFP fusion reporter to visualize and quantify microtubule organization in wild-type versus wdl mutant rhizoids.