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Investigating the Viral Suppressor HC-Pro Inhibiting Small RNA Methylation through Functional Comparison of HEN1 in Angiosperm and Bryophyte.

Sanobar, N., et al. · 2021 · Viruses   research

doi:10.3390/v13091837   PMID:34578418   PMC8473176

Linked genes (1)  1 core 0 peripheral

Gene ID Name Evidence / Role Function (this paper)
Mp3g16010 MpHEN1 experimental subject Codon-optimized full-length MpHEN1 (3053 bp) was cloned, expressed in E. coli, and his-MpHEN1 purified for in vitro methyltransferase assays. It methylated ds-syn-miR159 (67% methylated) but not ss-syn-miR159, like AtHEN1, confirming MpHEN1 as a small RNA methyltransferase; its activity was inhibited by HC-ProR in a dose-dependent manner.