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Improved clearing method contributes to deep imaging of plant organs.

Sakamoto, Y., et al. · 2022 · Communications Biology   research

doi:10.1038/s42003-021-02955-9   PMID:35013509   PMC8748589

Linked genes (2)  0 core 2 peripheral

Gene ID	Name	Evidence / Role	Function (this paper)
Mp3g17930	MpRSL1	experimental comparator	A pMpRSL1:NLS-tdTomato reporter line was cleared with iTOMEI and imaged; MpRSL1 promoter activity marked preapical regions of immature gemmae and rhizoid precursor cells, and the Mprsl1-1 mutant was examined for apical defects. Established as a useful marker for meristematic cells in liverwort.
Mp3g23400	MpEF1	experimental tool	MpEF1 promoter drove histone H2B-tdTomato to label nuclei in transgenic plants for iTOMEI deep-imaging demonstration.