Jahan, A., et al. · 2022 · Frontiers in Plant Science research
doi:10.3389/fpls.2022.952820 PMID:35968153 PMC9370073
| Gene ID | Name | Evidence / Role | Function (this paper) |
|---|---|---|---|
| Mp5g08310 | MpB3-6 | experimental subject | Ortholog of Arabidopsis ABI3 (Mapoly0086s0035/Mp5g08310), a key regulator of ABA-triggered gemma dormancy. Its transcript was significantly reduced in Mpark1/2ge and Mpark1/3ge but only slightly in Mpark1ge, placing it downstream of B3-Rafs. |
| Mp2g22820 | MpBZIP12 | experimental subject | Gene encoding an ABI5-like transcription factor; expression significantly reduced (-5.13-fold, FDR<0.05) in Mpark1/2ge but not Mpark1/3ge, potentially explaining the drastic loss of ABA-induced gene expression in that double mutant. |
| Mp8g15840 | MpCTR1 | experimental subject | B3 Raf kinase (Mapoly0079s0028/Mp8g15840; EDR1 and kinase domains, no PAS) studied functionally. Mpark3ge single mutant retained gemma dormancy; in Mpark1/3ge double mutants ABA-induced LEAL expression was variably reduced. |
| Mp8g15630 | MpCTR2 | experimental subject | B3 Raf kinase (Mapoly0079s0050/Mp8g15630; EDR1 and kinase domains, no PAS) studied functionally. Mpark2ge showed reduced sensitivity to 1 uM ABA but retained dormancy; co-disruption with MpARK1 strongly reduced ABA-induced gene expression and desiccation tolerance. |
| Mp8g16320 | MpCTR3 | experimental subject | A group B3 Raf kinase (Mapoly0154s0032/Mp8g16320, 1294 aa, with PAS, EDR1 and kinase domains) functionally studied here as the principal positive regulator of ABA signaling. Mpark1ge mutants lost gemma dormancy (rhizoids germinated in gemma cups) and showed reduced ABA-induced LEAL/MpABI3 expression; effects were amplified in double mutants. |
| Mp6g02960 | MpLEAL3 | experimental subject | LEA-like ABA-responsive gene (Mapoly0035s0082/Mp6g02960) quantified by qRT-PCR; ABA-induced expression greatly reduced in double mutants with variable reductions in single mutants. |
| Mp4g05760 | MpLEAL5 | experimental subject | LEA-like ABA-responsive gene (Mapoly0087s0015/Mp4g05760) quantified by qRT-PCR; ABA-induced expression significantly reduced in Mpark1/2ge. |
| Mp5g05120 | MpLEAL6 | experimental subject | LEA-like ABA-responsive gene (Mapoly0027s0114/Mp5g05120) quantified by qRT-PCR; ABA-induced expression severely reduced in Mpark1/2ge double mutant. |
| Mp5g15330 | MpMAPKKK12 | experimental subject | Group B2 Raf gene (Mapoly0071s0076/Mp5g15330; PAS and kinase domains, no EDR1) tested functionally. Restored ABA-induced expression less effectively than MpB2Raf1; single and double mutants showed wild-type-like ABA response. |
| Mp7g02280 | MpMAPKKK16 | experimental subject | Group B2 Raf gene (Mapoly0088s0059/Mp7g02280; PAS and kinase domains, no EDR1) tested functionally. Restored ABA-induced expression in P. patens with greater effect than MpB2Raf2, but Mpb2raf mutant ABA response resembled wild-type. |
| Mp4g09300 | MpLEAL1 | experimental comparator | LEA-like ABA-responsive marker gene (Mapoly0112s0030/Mp4g09300) quantified by qRT-PCR; ABA-induced expression severely reduced in Mpark1/2ge, indicating B3-Raf-dependent regulation of dehydration-protective transcripts. |
| Mp8g17460 | MpPYL1 | experimental comparator | Major PYR/PYL/RCAR ABA receptor; Mppyl1ge data compared to B3-Raf mutants. Most B3-Raf-regulated genes are under MpPYL1 control, but B3-Raf mutants showed stronger phenotypes than Mppyl1ge. |
| Mp3g23400 | MpEF1 | experimental tool | Reference gene (Mapoly0024s0116/Mp3g23400), ABA-unaffected, used for normalization in qRT-PCR. |
| — | MpABI1 | citation background | MpABI1 encoding a PP2C-A negative regulator of ABA signaling, mentioned as part of the core ABA pathway. |