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Plant autophagosomes mature into amphisomes prior to their delivery to the central vacuole.

Zhao, J., et al. · 2022 · Journal of Cell Biology   research

doi:10.1083/jcb.202203139   PMID:36260289   PMC9584626

Linked genes (3)  1 core 2 peripheral

Gene ID Name Evidence / Role Function (this paper)
MpCFS1 missing experimental subject Marchantia CFS1; mScarlet-MpCFS1 colocalized with MpATG8A/MpATG8B on autophagic structures and Mpcfs1 mutants were defective in GFP-ATG8 degradation, and MpCFS1 expressed in Arabidopsis colocalized with GFP-ATG8A. Shows CFS1 function in autophagosome maturation is conserved across plants.
Mp1g21590 MpATG8a experimental tool Autophagy marker isoform used as an mScarlet/GFP-tagged reporter that colocalizes with MpCFS1 and serves as the GFP-release degradation substrate in Marchantia.
Mp5g05930 MpATG8b experimental tool Autophagy marker isoform used as a tagged reporter colocalizing with mScarlet-MpCFS1 on autophagic structures in Marchantia thallus cells.