Tansley, C., et al. · 2023 · New Phytologist research
doi:10.1111/nph.18633 PMID:36660914 PMC10953335
| Gene ID | Name | Evidence / Role | Function (this paper) |
|---|---|---|---|
| Mp2g26670 | MpCIPK-B | experimental subject | CBL-interacting kinase in the AtCIPK24/SOS2 clade, the central subject of the study. Salt-downregulated, osmotic-upregulated; interacts with MpCBL-A/B in yeast and all MpCBLs in planta. CRISPR knockouts show increased salt sensitivity (chlorosis/death from 50 mM NaCl) but normal sorbitol tolerance, establishing it as essential for ionic salt-stress signalling. |
| Mp2g07750 | MpCBL-A | experimental tool | Calcineurin B-like protein analyzed by phylogeny, expression, and interaction assays. Groups with tonoplast Arabidopsis CBLs but lacks the localization motif; downregulated by salt yet upregulated by osmotic stress; interacts with both MpCIPKs in planta. |
| Mp4g00900 | MpCBL-B | experimental tool | Type I CBL with the MGCxxS/T plasma-membrane motif; modestly downregulated by salt and upregulated by osmotic stress; interacts with both MpCIPKs and Arabidopsis CIPK24 in yeast and in planta. |
| Mp5g19810 | MpCBL-C | experimental tool | Type I CBL proposed as the SOS3/AtCBL4 homologue; strongly salt-downregulated but osmotic-stress-insensitive, suggesting an ionic-stress role; interacts with both MpCIPKs in planta. |
| Mp1g05680 | MpCIPK-A | experimental tool | CBL-interacting kinase in a bryophyte-specific clade; salt-downregulated and osmotic-insensitive; binds all MpCBLs and Arabidopsis CBL4 in yeast and in planta, possibly via nonspecific interactions. |
| — | MpSOS1 missing | sequence background | Putative Na+/H+ antiporter; BLAST with AtSOS1 identifies two possible M. polymorpha homologues, proposed as downstream targets of MpCIPK-B, but phosphorylation/activation remains to be established. |