Takaoka, Y., et al. · 2024 · PNAS Nexus research
doi:10.1093/pnasnexus/pgae312 PMID:39139264 PMC11319934
| Gene ID | Name | Evidence / Role | Function (this paper) |
|---|---|---|---|
| — | MpMYC-Y missing | experimental subject | The actual MYC target tested in M. polymorpha; the stapled peptide 4 bound MpMYC-Y with high affinity (Kd 18 nM vs 830 nM for non-stapled peptide). Functional validation via the mpmycY knockout showed peptide 4 had almost no off-target effects, confirming MpMYC-Y as the selective target. |
| Mp7g02640 | MpEIN3 | experimental comparator | Off-target comparator ortholog of AtEIN3; peptide 4 bound MpEIN3 (Kd 89 nM) with no significant selectivity gain over the non-stapled peptide, and its downstream markers were not suppressed, contrasting with MpMYC-Y selectivity. |
| — | MpbHLH62 missing | experimental tool | MpMYC-mediated marker gene whose OPDA-induced expression was significantly suppressed by co-treatment with peptide 4, used as a readout of MYC inhibition. |
| Mp5g16510 | MpDIR39 | experimental tool | MpMYC-mediated marker gene; OPDA-induced expression was significantly suppressed by peptide 4, and suppression in the mpmycY mutant confirmed MYC-dependent specificity. Used as a readout. |
| Mp3g23400 | MpEF1 | sequence tool | Used as the reference gene for normalization in RT-qPCR of Marchantia seedlings. |
| Mp1g20040 | MpERF1 | experimental tool | MpEIN3-mediated marker gene; expression was activated by peptide 4 with OPDA, used as a readout of EIN3-branch activity. |
| Mp5g22160 | MpERF21 | experimental tool | MpEIN3-mediated marker gene; expression was activated by peptide 4 with OPDA relative to OPDA alone, used as a readout of EIN3-branch activity. |
| Mp5g21760 | MpPATATIN missing | experimental tool | MpMYC-mediated marker gene whose OPDA-induced expression was significantly suppressed by co-treatment with peptide 4, used as a readout of MYC inhibition. |
| Mp2g26590 | MpCOI1 | citation background | Cited in the evolutionary discussion that the COI1/JAZ co-receptor and MYC TF module establishes the JA signaling pathway arose more than 450 million years ago. Not experimentally manipulated. |
| — | MpMYC missing | experimental background | General reference to the MYC transcription factor in M. polymorpha jasmonate signaling; the two sex-chromosome-encoded paralogs MpMYC-X and MpMYC-Y are described as orthologs of Arabidopsis MYC TFs. |
| — | MpMYC-X missing | sequence background | One of two MYC genes encoded by the M. polymorpha sex chromosomes, identified as an ortholog of Arabidopsis MYC TFs with conserved function in activating the JA pathway. Cited as background, not directly assayed. |