Liang, W., et al. · 2024 · Plant Physiology research
doi:10.1093/plphys/kiae610 PMID:39514772 PMC11663715
| Gene ID | Name | Evidence / Role | Function (this paper) |
|---|---|---|---|
| Mp6g07600 | MpGH3A | experimental subject | Central gene of the study; CRISPR-Cas9 loss-of-function alleles (Mpgh3a-1ge, -2ge) were generated and shown to be unable to accumulate wound-induced dn-iso-OPDA amino-acid conjugates. |
| Mp2g26590 | MpCOI1 | experimental comparator | Mpcoi1-2 mutant analyzed; wound-induced dn-OPDA-aa conjugate accumulation was only slightly diminished, indicating a minor requirement of the COI1 pathway. |
| Mp2g14010 | MpGH3B | experimental comparator | Paralog contrasted with MpGH3A; not induced by OPDA and not transcriptionally regulated by dn-OPDA, so it does not contribute to conjugate formation. |
| — | MpMYCY | experimental comparator | Mpmycy mutant analyzed; like Mpcoi1 it showed only minor defects in dn-OPDA-aa conjugate accumulation. |
| Mp6g11010 | MpACTIN7 | experimental tool | Used as the reference gene (Mp6g11010) for RT-qPCR marker gene expression analysis. |
| Mp5g16920 | MpDIR33 | experimental tool | dn-OPDA-specific marker gene whose expression was measured; significantly higher in Mpgh3age plants than WT upon wounding. |
| Mp3g10660 | MpFAD5 | experimental tool | Mpfad5 mutant impaired in dn-iso-OPDA biosynthesis used to show conjugates derive mainly from the canonical dn-OPDA pathway. |
| — | MpHBLH4 missing | experimental tool | dn-OPDA-specific marker gene co-analyzed with MpDIR; expression significantly elevated in Mpgh3age plants versus WT upon wounding. |
| Mp6g17330 | MpDES6 | citation background | Cited as the Delta-6-desaturase required for hexadecatrienoic acid (16:3) biosynthesis in the hexadecanoid pathway; no experimental work here. |
| Mp1g20090 | MpILR | experimental background | Sole Marchantia amidohydrolase homolog; in vitro analyses (in discussion) showed it can accept different auxin conjugates as substrates, relevant to possible conjugate hydrolysis. |
| Mp6g06230 | MpJAZ | citation background | Listed only by accession (Mp6g06230); no experimental analysis reported in this paper. |