← Literature list

MurA- catalyzed synthesis of 5- enolpyruvylshikimate-3- phosphate confers glyphosate tolerance in bryophytes.

Caygill, S., et al. · 2024 · Proceeding of the National Academy of Sciences USA   research

doi:10.1073/pnas.2412997121   PMID:39527734   PMC11588093

Linked genes (3)  2 core 1 peripheral

Gene ID	Name	Evidence / Role	Function (this paper)
Mp5g14110	MpMurA	experimental subject	Main gene: CRISPR loss-of-function mutants are more glyphosate-sensitive than WT while overexpression increases tolerance, and the enzyme catalyzes transfer of the PEP enolpyruvyl moiety to S3P to produce EPSP. Heterologous expression in Arabidopsis confers glyphosate resistance, identifying MpMurA as the EPSP-synthesizing route conferring glyphosate tolerance in bryophytes.
Mp1g21490	MpMurF	experimental subject	Negative control: CRISPR loss-of-function MpmurF mutants show glyphosate sensitivity not significantly different from WT, demonstrating MpMurA-conferred tolerance is independent of chloroplast peptidoglycan biosynthesis.
Mp6g04140	MpEPSPS	experimental comparator	Recombinant M. polymorpha EPSPS was kinetically characterized; its glyphosate IC50 (9.60 uM) is not significantly different from A. thaliana EPSPS (14.49 uM), with near-identical predicted structure, showing tolerance is not attributable to a glyphosate-insensitive EPSPS.