Komatsu, A., et al. · 2025 · Nature Communications research
doi:10.1038/s41467-024-55728-3 PMID:39893162 PMC11787308
| Gene ID | Name | Evidence / Role | Function (this paper) |
|---|---|---|---|
| Mp5g09160 | MpDLP1 missing | experimental subject | Dienelactone hydrolase-like protein gene, one of three DEGs selected for further qPCR validation. Induced by (-)-GR24, characterized as a responsive gene of KAI2-dependent signaling. |
| Mp2g11710 | MpKAI2A | experimental subject | Putative receptor perceiving endogenous KAI2 ligands (KL) at the top of the signaling pathway studied here. Mpkai2a mutants were analyzed and showed altered MpLOG expression, placing KAI2-dependent signaling upstream of LOG-mediated cytokinin synthesis and gemma formation. |
| Mp2g04930 | MpKAI2B | experimental subject | One of the genes induced by (-)-GR24 treatment, examined by qPCR. MpKAI2B was activated even in the presence of cycloheximide, indicating it is a primary response gene of KAI2-dependent signaling. |
| Mp1g00270 | MpLOG | experimental subject | Central subject: cytokinin biosynthesis enzyme (LONELY GUY) whose expression is positively controlled by KAI2-dependent signaling. Mplog CRISPR mutants showed reduced endogenous cytokinin and reproductive defects rescued by exogenous cytokinins; cell-type-specific expression in gemma initials was shown via proMpLOG-EGFPNLS. |
| Mp1g02580 | MpMAX2 | experimental subject | F-box component acting downstream of KAI2 to trigger proteolysis of MpSMXL. Mpmax2 mutants were analyzed for MpLOG expression, confirming MAX2-dependent signaling controls LOG induction. |
| Mp3g06310 | MpSMXL | experimental subject | Suppressor protein degraded in a MpMAX2-dependent manner upon KL perception. Degradation-resistant MpSMXLd53 overexpression lines were used to confirm MpSMXL represses the downstream pathway controlling cytokinin synthesis and reproduction. |
| Mp6g11010 | MpACTIN7 | experimental tool | Actin gene used as a reference/housekeeping gene for normalizing qPCR expression data. |
| Mp3g25140 | MpAPT | experimental tool | Adenine phosphoribosyltransferase gene used as a reference/housekeeping gene for normalizing qPCR expression data. |
| — | missing | experimental background | Examined in GR24-response expression analysis but showed only a non-significant tendency toward induction; not a focus of the study's conclusions. |
| Mp7g05020 | MpEXL9 | experimental background | Examined in GR24-response expression analysis but showed only a non-significant tendency toward induction; not a focus of the study's conclusions. |