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Extensive N4 cytosine methylation is essential for Marchantia sperm function.

Walker, J., et al. · 2025 · Cell 188: doi: 10.1016/j.cell.2025.1003.1014   research

doi:10.1016/j.cell.2025.03.014   PMID:40209706

Linked genes (7)  2 core 5 peripheral

Gene ID Name Evidence / Role Function (this paper)
Mp6g18330 MpDN4MT1a experimental subject DNA N4-cytosine methyltransferase (Mp6g18330); one of two tandemly duplicated genes clustering with prokaryotic N4C MTases. Extensive N4-cytosine methylation it deposits is essential for Marchantia sperm function.
Mp6g18340 MpDN4MT1b experimental subject Tandem paralog (Mp6g18340) of MpDN4MT1a; N4-cytosine methyltransferase.
Mp6g08650 MpCMTa sequence comparator Chromomethylase; 5mC methyltransferase.
Mp5g09290 MpDNMT3b experimental comparator DNMT3-type (5mC) methyltransferase (Mp5g09290); compared to the N4C system.
MpROS1 missing sequence comparator DEMETER/ROS1-type DNA demethylase.
Mp3g23400 MpEF1 experimental tool Reference gene for qPCR.
MpDN4MT1 missing experimental background MpDN4MT1 N4-cytosine methyltransferase (deletion analysis)