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Semi-automated workflow for high-throughput Agrobacterium-mediated plant transformation.

Annese D, Romani F, Grandellis C, Ives L, Frangedakis E, Buson FX, Molloy JC, Haseloff J. · 2025 · The Plant journal : for cell and molecular biology   research

doi:10.1111/tpj.70118   PMID:40220013   PMC11993085

Linked genes (2)  0 core 2 peripheral

Gene ID Name Evidence / Role Function (this paper)
MpEF1a missing experimental tool The MpEF1a promoter (proMpEF1a) was used to drive an mVenus-N7 fluorescent nuclear marker in transformation reporter constructs to validate the high-throughput Agrobacterium-mediated transformation workflow.
MpUBE2 missing experimental tool The MpUBE2 promoter (proMpUBE2) was used to drive an mScarlet-Lti6b membrane marker in reporter constructs for the transformation workflow; it showed no activity in N. benthamiana.