Zhou Y, Hamiaux C, Andre CM, Cooney JM, Schwinn KE, van Klink JW, Bowman JL, Davies KM, Albert NW. · 2025 · The New phytologist research
doi:10.1111/nph.70194 PMID:40325841 PMC12138178
| Gene ID | Name | Evidence / Role | Function (this paper) |
|---|---|---|---|
| Mp6g05290 | MpCHI | experimental subject | Chalcone isomerase. Mpchi mutants (and Mpchi-chil double mutants) were analyzed by RNAseq in this work for comparison with Mppr10.5; CHI was among flavonoid genes downregulated in Mppr10.5. |
| Mp5g01410 | MpCHIL | experimental subject | Chalcone isomerase-like. Mpchil and Mpchi-chil double mutants were generated/analyzed here, showing limited differential expression; CHIL was downregulated in Mppr10.5 mutants. |
| Mp8g00860 | MpPR10.5 | experimental subject | Primary subject. The Mp8g00860 ORF (149 aa) was classified in the PR10 family and named MpPR10.5. CRISPR/Cas9 mutants and metabolite-binding assays showed MpPR10.5 binds and protects naringenin chalcone; loss of function downregulates flavonoid biosynthesis genes, demonstrating it promotes flavonoid biosynthesis. |
| Mp1g01300 | MpHDAC1 | experimental tool | Reference/housekeeping gene used to normalize qPCR/expression data. |
| Mp2g05850 | MpAS1 paper
MpAUS nomenclature |
experimental background | Aureusidin synthase; downregulated in Mppr10.5 RNAseq. |
| Mp6g00020 | MpC4H paper
MpC4H1 nomenclature |
experimental background | Highest-abundance C4H, transcript level unchanged in Mppr10.5. |
| Mp8g05080 | MpC4H3 | experimental background | Cinnamate 4-hydroxylase; downregulated in Mppr10.5 RNAseq. |
| — | MpCHS13 missing | experimental background | Chalcone synthase downregulated in Mppr10.5 RNAseq. |
| — | MpCHS17 missing | experimental background | Highest-abundance CHS, transcript level unchanged in Mppr10.5. |
| — | MpCHS2 missing | experimental background | Chalcone synthase downregulated in Mppr10.5 RNAseq. |
| Mp1g20080 | MpDBR | experimental background | Bis-bibenzyl biosynthetic gene, unaffected in Mppr10.5. |
| — | MpMYB14 missing | citation background | Auronidin/flavonoid transcriptional regulator; cited prior 35S:MYB14 and Mpmyb14 lines showing MpPR10.5 is a MpMYB14 target. |
| Mp4g14160 | MpPAL-like2 | experimental background | PAL-like gene downregulated in Mppr10.5 RNAseq. |
| Mp1g10150 | MpPAL1 | experimental background | Phenylalanine ammonia-lyase; downregulated in Mppr10.5 RNAseq. |
| — | MpPKR missing | experimental background | Bis-bibenzyl biosynthetic gene, unaffected in Mppr10.5. |
| Mp5g07330 | MpPR10.3 | citation background | PR10 family member; cited UVB-responsive expression pattern. |
| Mp8g08940 | MpPR10.9 | citation background | PR10 family member; cited UVB-responsive expression pattern. |
| Mp7g14930 | MpSTCS1A | experimental background | Bis-bibenzyl biosynthetic gene, unaffected in Mppr10.5. |