Kanaris M, Lee J, Chang B, Christendat D. · 2025 · Molecular biology and evolution research
doi:10.1093/molbev/msaf129 PMID:40452216 PMC12203367
| Gene ID | Name | Evidence / Role | Function (this paper) |
|---|---|---|---|
| Mp3g21830 | MpSK1 | experimental subject | MpSK (Mp3g21830) was identified as a shikimate kinase homolog; enzyme kinetic assays showed it phosphorylates shikimate, confirming it is a functional SK. |
| Mp6g03600 | MpSK2 | experimental subject | CRISPR/Cas9 disruption of MpSKL1 (Mp6g03600) caused pigment-defective phenotypes, reduced growth and pale tissue, with abnormal chloroplast/thylakoid morphology and reduced chlorophyll a/b. Heterologous AtcTP-delta80MpSKL1 also rescued chloroplast biogenesis in Arabidopsis skl1-8, supporting a conserved role in chloroplast biogenesis. |
| Mp3g23400 | MpEF1 | experimental tool | Used as a housekeeping/internal control gene for expression analysis. |
| Mp3g07860 | missing | sequence background | Third M. polymorpha SK gene homolog, identified by sequence as separating among SKL2 sequences; not functionally characterized here. |