Alisha A, Jarmolowski A, Szweykowska-Kulinska Z, Sierocka I. · 2026 · The Plant journal : for cell and molecular biology research
doi:10.1111/tpj.70755 PMID:41840856 PMC12993094
| Gene ID | Name | Evidence / Role | Function (this paper) |
|---|---|---|---|
| Mp8g11450 | MpAP2L1 | experimental subject | MpANT/MpPLT, a meristem-development TF, showed the strongest downregulation among tested genes in Mpspl3 knockout mutants by qPCR in this study. |
| Mp1g07070 | MpARF3 | experimental subject | MpARF3 (AUXIN RESPONSE FACTOR3) transcript level was unchanged in Mpspl3 knockout mutants by qPCR in this study, indicating it is not regulated by MpSPL3 despite a hypothesized role in inhibiting the reproductive transition. |
| Mp3g23300 | MpBNB | experimental subject | MpBNB (BONOBO), involved in germ cell specification, was strongly downregulated in Mpspl3 knockout (and overexpression) plants by qPCR in this study. |
| Mp2g23000 | MpBZR3 | experimental subject | MpBZR3 (BRASSINAZOLE-RESISTANT 3) was strongly downregulated in Mpspl3 knockout and overexpression mutants by qPCR here, linking MpSPL3 to far-red-responsive reproductive programs. |
| Mp2g02750 | MpCKI1 | experimental subject | MpCKI1 (CYTOKININ-INDEPENDENT 1 histidine kinase), required for proper antheridiophore morphology, was significantly downregulated in Mpspl3 knockout mutants by qPCR here. |
| Mp7g09740 | MpGARP8 | experimental subject | MpGLK (GOLDEN2-LIKE) mRNA was downregulated in Mpspl3 knockout mutants by qPCR here; it normally activates MpMAX2 to regulate gemma cup timing. |
| Mp2g11710 | MpKAI2A | experimental subject | MpKAI2A, controlling gemma cup formation and gemma initiation, was downregulated in Mpspl3 knockout mutants by qPCR in this study. |
| Mp2g20695 | MpLRL | experimental subject | MpLRL (LJRHL-LIKE), which stabilizes MpBNB, was significantly downregulated in Mpspl3 knockout mutants by qPCR in this study. |
| Mp3g05470 | MpMIR529C | experimental subject | SPL-family gene downregulated in Mpspl3 knockout plants here, indicating MpSPL3 acts upstream of the miR529c-MpSPL2 module driving the vegetative-to-reproductive phase transition. |
| Mp7g17540 | MpROP | experimental subject | MpROP (RHO OF PLANTS) mRNA was downregulated in Mpspl3 knockout mutants by qPCR here, linking MpSPL3 to rhizoid/meristem-related programs. |
| Mp1g10020 | MpSPL1 | experimental subject | SPL-family gene found significantly downregulated in Mpspl3 knockout mutants in this study, placing it downstream of MpSPL3 in coordinating meristem dormancy. |
| Mp1g13640 | MpSPL3 | experimental subject | Primary subject. The SPL-family TF MpSPL3 produces two splice isoforms (MpSPL3.1 longer by 183 nt due to in-frame intron 3 retention; MpSPL3.2). amiR knockout of both transcripts causes complete loss of gametangiophore formation, while overexpression of MpSPL3.1 delays and reduces gametangiophore production, showing MpSPL3 coordinates vegetative growth and the reproductive phase transition. |
| Mp8g11850 | MpSPL4 | experimental subject | AtSPL7-like SPL gene whose expression was downregulated in Mpspl3 knockout mutants under white light in this study, placing it downstream of MpSPL3. |
| Mp3g23400 | MpEF1 | experimental tool | MpEF1α was used as the reference gene for RT-qPCR normalization in this study. |
| Mp1g26670 | MpMIR160 | experimental tool | The MpmiR160 precursor backbone was used as the scaffold for the artificial miRNA designed to knock down MpSPL3. |
| Mp1g02580 | MpMAX2 | citation background | Cited from prior work as a gene activated by MpGLK to regulate gemma cup timing. |
| Mp6g07990 | Mpo-MR-13 missing | citation background | Liverwort-specific miRNA (MpmiR11671) cited as regulating MpSPL1 in meristem dormancy. |
| Mp3g06310 | MpSMXL | citation background | Cited from prior work as part of the KAI2-dependent signaling pathway. |
| Mp8g08780 | MpYUC2 | citation background | Mentioned as background in the context of auxin biosynthesis gene regulatory networks. |