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Reconstruction of a Bis(bibenzyl) Biosynthetic Pathway through Analysis of 4-Coumarate:CoA Ligase and Double-Bond Reductase in Marchantia polymorpha.

Kobayashi Y, Tachibana M, Kimura N, Hatada M, Koeduka T, Ohta A, Shimizu BI, Nomura T, Kato Y, Ishizaki K, Mizutani M, Kubo H, Takanashi K. · 2026 · Plant & cell physiology   research

doi:10.1093/pcp/pcag049   PMID:42011935

Linked genes (3)  2 core 1 peripheral

Gene ID	Name	Evidence / Role	Function (this paper)
—	Mp4CL3 missing	experimental subject	Mp4CL3, a 4-coumarate:CoA ligase upregulated in a high bis(bibenzyl) line, was characterized via E. coli enzyme assays and showed esterification activity toward both p-coumaric acid and dihydro-p-coumaric acid, producing their corresponding CoA thioesters in the bis(bibenzyl) pathway.
—	MpDBR1 missing	experimental subject	MpDBR1, a double-bond reductase, showed reduction activity specific to p-coumaroyl-CoA (not p-coumaric acid); CRISPR/Cas9 knockout significantly reduced bis(bibenzyl) content, evidencing a route from p-coumaric acid to dihydro-p-coumaroyl-CoA via p-coumaroyl-CoA.
—	MpMYB2 missing	experimental background	MpMYB2 overexpression line, with high bis(bibenzyl) accumulation, was the genetic background in which Mp4CL3 and MpDBR1 were found upregulated.