Kopischke, S., et al. · 2017 · Plant Methods research
doi:10.1186/s13007-017-0167-5 PMID:28360929 PMC5370431
| Gene ID | Name | Evidence / Role | Function (this paper) |
|---|---|---|---|
| Mp8g11800 | MpNOP1 | experimental subject | Selected as the TALEN knockout target because its mutant lacks air chamber formation (an easily traceable phenotype); both TALEN approaches achieved >20% targeting efficiency with deletions of 4 nt to ~2000 nt across 22 sequenced T1 lines. Two mutants carried in-frame indels revealing novel Mpnop1-related phenotypes, with stable inheritance across generations. |
| Mp3g23400 | MpEF1 | experimental tool | Its characterized, strongly expressed endogenous promoter was used in both binary vectors to drive TALEN expression in Marchantia tissues including meristematic regions. |