Mp8g11800



Description

Annotation

Database ID Description
Coils Coil Coil
SMART SM00504 Ubox_2
SUPERFAMILY SSF57850 RING/U-box
CDD cd21037 MLKL_NTD
SUPERFAMILY SSF48371 ARM repeat
Gene3D G3DSA:3.30.40.10 Zinc/RING finger domain, C3HC4 (zinc finger)
SMART SM00185 arm_5
Pfam PF04564 U-box domain
Pfam PF19584 MCAfunc domain
ProSiteProfiles PS51698 U-box domain profile.
Gene3D G3DSA:1.25.10.10 -
CDD cd16664 RING-Ubox_PUB
PANTHER PTHR45958 RING-TYPE E3 UBIQUITIN TRANSFERASE
KEGG K08332 vacuolar protein 8
KOG KOG4224 Armadillo repeat protein VAC8 required for vacuole fusion, inheritance and cytosol-to-vacuole protein targeting; C-term missing; [U]
KOG KOG0166 Karyopherin (importin) alpha; N-term missing; C-term missing; [U]
MapolyID Mapoly0008s0036 -
GO GO:0004842 ubiquitin-protein transferase activity
GO GO:0005515 protein binding
GO GO:0016567 protein ubiquitination

Nomenclature

Gene symbol Product Transcript ID Status
MpNOP1 Plant U-box E3 Ubiquitin Ligase NOP1 Published

Associated Literature (AI-based literature mining, Beta-version)

Plant U-box E3 Ubiquitin Ligase NOP1; NOP1 has an essential role in schizogenous intercellular space formation in Marchantia polymorpha.NOP1 encodes a Plant U-box (PUB) E3 ubiquitin ligase carrying tandem ARMADILLO (ARM) repeats in the C terminus. An in vitro ubiquitination assay indicated that the NOP1 protein possesses E3 ubiquitin ligase activity in a U-box-dependent manner. Confocal microscopy and biochemical analysis showed that NOP1 was localized to the plasma membrane. (from nomenclature)

2 core 4 peripheral

Sugano, S. S., et al. (2018) · PLoS ONE  research experimental subject
Targeted to assess editing efficiency via the easily scored transparent-thallus (air-chamber-less) phenotype; 87.5% of 32 T1 plants were mutated. Used to test guide length (17nt or fewer dramatically less efficient) and to induce 0.5/1.5/4.5kb deletions with paired gRNAs; no off-target mutations.
Kopischke, S., et al. (2017) · Plant Methods  research experimental subject
Selected as the TALEN knockout target because its mutant lacks air chamber formation (an easily traceable phenotype); both TALEN approaches achieved >20% targeting efficiency with deletions of 4 nt to ~2000 nt across 22 sequenced T1 lines. Two mutants carried in-frame indels revealing novel Mpnop1-related phenotypes, with stable inheritance across generations.
Lim, C. J., et al. (2024) · New Phytologist  research citation background
Alternative name for MpPUB24/NOPPERABO1, previously reported to control formation of intercellular spaces.
Ishizaki, K. (2015) · Frontiers in Plant Science  review citation background
NOPPERABO1; PUB E3 ligase required for intercellular space formation
Althoff, F., et al. (2022) · Fronteirs in Plant Science  research citation comparator
Plant U-box gene controlling air pore formation

Expression Level powered by MBEX

Link to the original image in MBEX

Transcript models

Transcript ID Location Sequences Extract region
Mp8g11800.1 chr8:15294953..15302422 (-) Gene /  mRNA /  CDS /  Protein
FASTA / GenBank (with flanking bases)
Mp8g11800.2 chr8:15294953..15302422 (-) Gene /  mRNA /  CDS /  Protein
FASTA / GenBank (with flanking bases)

Gene structure:

Sequences:

Gene UTR + CDS + intron

FASTA

mRNA UTR + CDS

FASTA

CDS

FASTA

Protein

FASTA

Gene structure:

Sequences:

Gene UTR + CDS + intron

FASTA

mRNA UTR + CDS

FASTA

CDS

FASTA

Protein

FASTA


MarpolBase / Genome Informatics Lab. NIG.