Lim, C. J., et al. · 2024 · New Phytologist research
doi:10.1111/nph.20169 PMID:39387122 PMC11579444
| Gene ID | Name | Evidence / Role | Function (this paper) |
|---|---|---|---|
| Mp5g19550 | MpEXO70.1 missing | experimental subject | Identified as the direct substrate of MpPUB9 among exocyst paralogs. MpPUB9 interacts with and ubiquitinates MpEXO70.1, promoting its turnover; protein stability measured under salt stress and overexpression lines analyzed. |
| — | MpPUB10 missing | experimental subject | Closely related U-box gene functionally tested for contrast: gain/loss-of-function mutants showed no prominent thallus growth-angle phenotype and the protein did not interact with EXO70 paralogs in yeast, underscoring MpPUB9's specificity. |
| — | MpPUB24 missing | experimental subject | Studied U-box ligase (= MpNOP1/NOPPERABO1) shown to retain intact enzyme activity; used in functional/sequence comparison of the U-box domain to characterize the MpPUB family. |
| — | MpPUB9 missing | experimental subject | Central subject: U-box E3 ubiquitin ligase rapidly induced by high salinity and dehydration. Gain/loss-of-function mutants and overexpressors made; in vitro E3 ligase activity confirmed. Acts as positive regulator of salt tolerance by ubiquitinating and promoting turnover of MpEXO70.1. |
| — | MpCPS missing | citation comparator | Cited as required for far-red-induced hyponastic growth; expression unchanged in MpPUB9 overexpressors. |
| Mp1g14780 | MpEXO70.2 missing | experimental comparator | Exocyst paralog (Mp1g14780) tested as comparator; no interaction with MpPUB9 detected and localization examined. |
| Mp1g02880 | MpEXO70.3 missing | experimental comparator | Exocyst paralog (Mp1g02880) tested as comparator; interacted only very weakly with MpPUB9 in yeast. |
| Mp2g11710 | MpKAI2A | citation comparator | Karrikin-signaling gene cited; loss-of-function causes curved upward growth; expression unchanged in MpPUB9 lines. |
| Mp2g04930 | MpKAI2B | citation comparator | Karrikin-signaling gene cited alongside MpKAI2A; expression unchanged in MpPUB9 overexpressors. |
| Mp1g02580 | MpMAX2 | citation comparator | Karrikin-signaling gene cited with MpKAI2A/B; expression unaltered in MpPUB9 lines. |
| Mp5g05140 | MpPIN2 | citation comparator | Auxin transporter cited as affecting thallus growth angle; its expression not significantly changed in MpPUB9 overexpressors. |
| — | MpUBQ10 missing | experimental tool | Used as the reference/normalization gene for qRT-PCR of MpPUB9 expression. |
| Mp4g08580 | missing | sequence background | Added to the list of putative MpPUB genes based on sequence conservation. |
| Mp5g12010 | MpCOP1 | citation background | RING-type E3 ligase (Mp5g12010) cited and excluded from the PUB protein classification. |
| Mp8g11800 | MpNOP1 | citation background | Alternative name for MpPUB24/NOPPERABO1, previously reported to control formation of intercellular spaces. |