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DRP3 and ELM1 are required for mitochondrial fission in the liverwort Marchantia polymorpha.

Nagaoka, N., et al. · 2017 · Scientific Reports   research

doi:10.1038/s41598-017-04886-0   PMID:28676660   PMC5496855

Linked genes (3)  3 core 0 peripheral

Gene ID Name Evidence / Role Function (this paper)
Mp2g24350 MpDRP3 missing experimental subject Single-copy dynamin-related protein homologue studied by RT-PCR (two splice variants MpDRP3s/l), Citrine-fusion localization to mitochondrial constriction sites, and knockout (Mpdrp3). Mpdrp3 mutants showed severe growth retardation and blocked mitochondrial fission with elongated mitochondria, identifying MpDRP3 as required for mitochondrial fission.
Mp6g18400 MpELM1 missing experimental subject Single-copy ELM1 homologue studied by transient-expression localization (MpELM1-Citrine surrounding mitochondria) and knockout (Mpelm1). Mpelm1 showed growth retardation and elongated mitochondria resembling Mpdrp3, with complementation restoring morphology, identifying MpELM1 as required for mitochondrial fission.
Mp5g02260 MpFIS1 missing experimental subject Single-copy FIS1 homologue knocked out (Mpfis1); Citrine-MpFIS1 localized to mitochondrial surface and peroxisome-like bodies but Mpfis1 had no growth/developmental defects, indicating MpFIS1 has no major role in mitochondrial fission.