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Origin and evolution of the nuclear auxin response system.

Mutte, S. K., et al. · 2018 · eLIFE   research

doi:10.7554/elife.33399   PMID:29580381   PMC5873896

Linked genes (5)  1 core 4 peripheral

Gene ID Name Evidence / Role Function (this paper)
Mp1g12750 MpARF1 sequence subject Class A-ARF of M. polymorpha; homology model of its DBD was generated and compared to other ARFs. Analysis showed dimerization residues conserved in (proto-)A/B-ARFs including MpARF1. Cited arf mutant work indicating A-ARF role in auxin response.
Mp1g07070 MpARF3 experimental subject Single C-ARF of M. polymorpha; CRISPR/Cas9 loss-of-function mutants generated. arf3 mutants showed dramatic developmental defects with gemmae arrested before maturation. Auxin-responsive genes responded similarly in WT and mutants, indicating C-ARF targets differ from A-ARF.
Mp2g26590 MpCOI1 sequence subject M. polymorpha COI1-like jasmonate receptor homolog; homology-modeled alongside MpTIR1 using A. thaliana COI1 crystal structure as template for binding-residue comparison.
Mp6g02750 MpTIR1 sequence subject M. polymorpha TIR1/AFB-like auxin receptor; homology model built using A. thaliana TIR1 crystal structure as template to infer hormone-binding residues.
Mp4g11820 MpARF2 sequence comparator Class A/B-ARF of M. polymorpha included in homology modeling and used to show conservation of dimerization residues among A/B-ARFs.