Wachananawat, B., et al. · 2020 · Frontiers in Plant Science research
doi:10.3389/fpls.2020.00997 PMID:32714362 PMC7343896
| Gene ID | Name | Evidence / Role | Function (this paper) |
|---|---|---|---|
| Mp1g15230 | MpRRT1 | experimental subject | Genome locus of MpRRT1, the central gene of this study; its cDNA was amplified by PCR and expressed as recombinant protein in N. benthamiana. The encoded enzyme shows RG-I rhamnosyltransferase activity, localizes at the cis-Golgi, and CRISPR knockouts were generated. |
| Mp1g10780 | MpRRT3 | experimental subject | Genome locus of MpRRT3; recombinant protein expressed as a FLAG-tagged fusion in tobacco exhibited the same RG-I rhamnosyltransferase activity as MpRRT1, and it falls in the RRT clade of GT106. |
| Mp1g08030 | MpSYP3 | experimental tool | Cis-Golgi marker (pro35S:MpSYP3-Citrine) that co-localized with MpRRT1-tagRFP, establishing MpRRT1's cis-Golgi localization; not characterized itself. |
| Mp2g14140 | MpSYP4 | experimental tool | Trans-Golgi network marker (pro35S:MpSYP4-Citrine) co-transformed with MpRRT1-tagRFP as a localization reference; not characterized itself. |
| Mp1g18750 | MpUSE1A | experimental tool | Endoplasmic reticulum marker (pro35S:MpUSE1A-Citrine) co-transformed with MpRRT1-tagRFP to compare subcellular localization; not characterized itself. |
| — | missing | sequence background | A predicted gene located -0.7 to -3.9 kbp upstream of the MpRRT1 gene per MarpolBase, used as a reference landmark for promoter design. |