Liu, S., et al. · 2021b · Genome Biology research
doi:10.1186/s13059-021-02476-y PMID:34465381 PMC8408974
| Gene ID | Name | Evidence / Role | Function (this paper) |
|---|---|---|---|
| Mp7g12670 | MpBMI1 | experimental subject | Core PRC1 RING-finger E3 ligase (Mp7g12670) identified by BLAST; CRISPR knockout combined with Mpbmi1l caused strongly reduced growth and gemma-cup defects, global H2Aub loss by western blot, and altered H2Aub/H3K27me3 by ChIP-seq, showing it deposits H2Aub for Polycomb gene repression. |
| Mp6g09730 | MpBMI1L missing | experimental subject | Second BMI1 homolog (Mp6g09730) with only a RAWUL domain; functions redundantly with MpBMI1, as Mpbmi1/1l double knockouts were more severely affected than single mutants and lost H2Aub, establishing it as a PRC1 component required for H2A ubiquitination. |
| Mp3g02370 | MpH2A | experimental subject | Single canonical H2A whose K115/K116/K119 were mutated to arginine via CRISPR; western blot confirmed reduced H2Aub, ChIP-seq/RNA-seq showed H2Aub required for H3K27me3 at genes and TEs with thallus and gemmae-dormancy phenotypes, demonstrating the three lysines are redundantly ubiquitinated in vivo. |
| — | MpRING missing | citation comparator | PRC1 catalytic component mentioned only in discussion, with possible BMI1-independent catalytic activity inferred from Arabidopsis AtRING1A/B. |