Chini, A., et al. · 2021 · Plant Physiology research
doi:10.1093/plphys/kiab369 PMID:34618088 PMC8566257
| Gene ID | Name | Evidence / Role | Function (this paper) |
|---|---|---|---|
| Mp2g00930 | MpBHLH4 | experimental subject | bHLH14 transcription factor, OPDA-inducible marker; qPCR shows OPDA induction blocked by J4, evidencing J4 antagonism in Marchantia. |
| Mp2g26590 | MpCOI1 | experimental subject | F-box jasmonate co-receptor of M. polymorpha, expressed/purified in insect cells with MpASK1. dn-OPDA induces its interaction with MpJAZ in pull-down assays, and the antagonist J4 directly blocks this Mp receptor complex, showing conserved JA perception. |
| Mp6g06230 | MpJAZ | experimental subject | JAZ-domain repressor of M. polymorpha produced as recombinant MBP-MpJAZ; dn-OPDA induces MpCOI1-MpJAZ interaction in vitro, which J4 antagonizes. |
| Mp5g16920 | MpDIR33 | experimental comparator | Dirigent-like OPDA-responsive marker gene; induced by 10 uM OPDA in Tak-1 plants, induction suppressed by J4 pretreatment. |
| Mp5g21220 | MpPAT | experimental comparator | OPDA-responsive marker gene assayed by qPCR alongside MpDIR/MpBHLH4; J4 dampens its OPDA induction. |
| — | MpACT missing | experimental tool | Actin gene used as housekeeping reference for qRT-PCR normalization. |
| — | MpASK1 missing | experimental tool | SKP1-like adapter co-expressed and purified with MpCOI1 in insect cells to reconstitute the functional Mp receptor complex for pull-down assays. |