Iwasaki, M., et al. · 2021a · Current Biology research
doi:10.1016/j.cub.2021.10.023 PMID:34735792 PMC8699743
| Gene ID | Name | Evidence / Role | Function (this paper) |
|---|---|---|---|
| MpUg00370 | MpBPCU | experimental subject | A U chromosome-linked BASIC PENTACYSTEINE transcription factor gene identified as the master sex determinant ("Feminizer") in M. polymorpha. Two independent bpcU mutants formed male sex organs, showing BPCU is necessary and sufficient for female sex determination. It acts by directly regulating the autosomal FGMYB/SUF locus, repressing the SUF lncRNA via chromatin modification. |
| MpVg00350 | MpBPCV | experimental subject | V chromosome gametolog of BPCU; both share a conserved function required to induce sex organ development. bpcV haploid mutants failed to induce sex organ formation, and bpcU mutants complemented with BPCV developed male sex organs instead of female. |
| Mp1g17210 | MpFGMYB | experimental subject | Autosomal sex-determining locus (FEMALE GAMETOPHYTE MYB) that promotes female differentiation and is positively regulated by BPCU. Its expression correlated with morphological sex, and fgmyb mutations suppressed the feminization induced by gBPCU. |
| Mp1g17205 | MpSUF missing | experimental subject | Antisense long non-coding RNA (SUPPRESSOR OF FEMINIZATION) transcribed from a promoter 3' of FGMYB. BPCU directly represses SUF through chromatin modification, relieving FGMYB from suppression; bpcU mutants showed increased SUF and decreased FGMYB expression. |
| Mp5g13880 | missing | experimental tool | Female-specifically expressed gene used as a female marker in expression analyses. |
| Mp7g02820 | missing | experimental tool | Female-specifically expressed gene used as a female marker in expression analyses. |
| Mp3g23400 | MpEF1 | experimental tool | Ubiquitously expressed gene (Mp3g23400) used as an internal control reference in expression analyses. |
| Mp6g01560 | MpLC7 | experimental tool | Male-specifically expressed gene (Mp6g01560) used as a male marker in expression analyses. |
| Mp3g14390 | MpPRM | experimental tool | Male-specifically expressed gene (Mp3g14390) used as a male marker in expression analyses. |