Norizuk, T., et al. · 2022 · Autophagy research
doi:10.1080/15548627.2022.2096396 PMID:35786352 PMC9542840
| Gene ID | Name | Evidence / Role | Function (this paper) |
|---|---|---|---|
| Mp7g03210 | MpATG13 | experimental subject | Core autophagy-related gene. Knockout mutants retained a larger number of mitochondria in spermatozoids than wild-type, evidencing autophagy's requirement for organelle reorganization. |
| Mp1g00090 | MpATG14 | experimental subject | Core autophagy-related gene. Mutants lacking this gene were analyzed and showed retention of excess mitochondria in spermatozoids, confirming autophagy's contribution to mitochondrial reduction. |
| Mp1g12840 | MpATG5 | experimental subject | Core autophagy-related gene. Knockout mutants were generated and analyzed, showing spermatozoids retain more mitochondria than wild-type, demonstrating autophagy is required for reducing mitochondrial number during spermiogenesis. |
| Mp2g07850 | MpATG7 | experimental subject | Core autophagy-related gene. Loss-of-function mutants were analyzed alongside other core ATG genes; mutant spermatozoids retained excess mitochondria, supporting autophagy's role in organelle reduction. |
| Mp2g24350 | MpDRP3 missing | experimental tool | A mitochondrial dynamin-related protein; its dominant-negative form was expressed to inhibit mitochondrial fission, yielding spermatozoids with a single mitochondrion. Used as a genetic tool to probe mitochondrial fission's role in organelle reorganization. |