Liang, Y., et al. · 2022 · Science Advances research
doi:10.1126/sciadv.abo7737 PMID:35960801 PMC9374346
| Gene ID | Name | Evidence / Role | Function (this paper) |
|---|---|---|---|
| Mp5g16260 | MpAOS2 | experimental subject | Oxylipin biosynthesis gene (Mp5g16260) whose wound-induced transcript levels are MpERF15-dependent, being significantly lower in Mperf15ko. |
| Mp2g26760 | MpASA1 missing | experimental subject | Auxin-biosynthesis (anthranilate synthase) gene acting downstream of MpERF15; its wound-induced expression is significantly reduced in Mperf15ko at 4 h post-wounding by RT-qPCR. |
| Mp2g26590 | MpCOI1 | experimental subject | Oxylipin (COI1) receptor; MpERF15 acts both upstream and downstream of it. MpERF15 expression is strongly reduced in Mpcoi1-2 mutants, and MpERF15OE phenotypes are lost in the Mpcoi1-2 background, placing MpCOI1 in the feedback loop. |
| Mp7g06220 | MpDIR17 | experimental subject | Allene oxide cyclase (Mp7g06220) catalyzing a key OPDA/dn-OPDA biosynthesis step. Mpaocko mutants regenerate slowly, a defect rescued by OPDA treatment; its wound-induced expression is reduced in Mperf15ko and Mpcoi1-2. |
| Mp5g16520 | MpDIR38 | experimental subject | Dirigent-like protein gene (Mp5g16520), a previously known wound-inducible marker confirmed by RT-qPCR to be MpERF15-dependent. |
| Mp5g16470 | MpDIR42 | experimental subject | Dirigent-like protein gene belonging to the wound-inducible, MpERF15-dependent gene cluster identified by RNA-seq. |
| — | MpDIR43 missing | experimental subject | Dirigent-like protein gene belonging to the wound-inducible, MpERF15-dependent gene cluster identified by RNA-seq. |
| Mp7g09350 | MpERF15 | experimental subject | Wound-activated ERF transcription factor that is instantly induced after wounding and is essential for gemmaling regeneration. Knockouts (Mperf15ko) show regeneration defects while overexpression lines produce extra apical notches and ectopic meristems. Drives a positive oxylipin-biosynthesis feedback loop, operating both upstream and downstream of MpCOI1. |
| Mp5g06970 | MpERF20 | experimental subject | MpERF20/LAXR (Mp5g06970), a late wound-inducible factor (peak ~6 h) acting downstream of MpERF15; wound inducibility partly depends on MpCOI1, MpAOC and MpERF15, and expression is elevated in MpERF15OE thalli. |
| Mp6g06230 | MpJAZ | experimental subject | The single M. polymorpha JAZ repressor, phylogenetically closest to subgroup V; shown by yeast two-hybrid to interact with MpERF15 (and with Arabidopsis JAZ3/4/9). |
| Mp2g00660 | MpLOX1 | experimental subject | Lipoxygenase, wound-inducible in an MpERF15-dependent manner and induced in MpERF15OE lines; likely acts in the dn-OPDA biosynthetic pathway. |
| Mp5g21680 | MpLOX4 | experimental subject | Lipoxygenase, wound-inducible in an MpERF15-dependent manner; repressed in MpERF15OE lines and likely active in the OPDA biosynthetic pathway. |
| Mp6g11010 | MpACTIN7 | experimental tool | Constitutive actin gene (Mp6g11010) used as a reference gene for RT-qPCR normalization. |
| Mp3g23400 | MpEF1 | sequence tool | Constitutive ELONGATION FACTOR 1alpha promoter (Mp3g23400) used to drive overexpression constructs and as an RT-qPCR reference gene. |
| Mp8g08780 | MpYUC2 | experimental tool | Used as a proMpYUC2:GUS reporter line to mark apical notches/meristematic activity, with more GUS dots in MpERF15OE than WT. |