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Polycomb-mediated repression of paternal chromosomes maintains haploid dosage in diploid embryos of Marchantia.

Montgomery, S. A., et al. · 2022 · eLife   research

doi:10.7554/elife.79258   PMID:35996955   PMC9402228

Linked genes (8)  4 core 4 peripheral

Gene ID	Name	Evidence / Role	Function (this paper)
Mp5g17740	MpE(z)2	experimental subject	PRC2 catalytic subunit; CRISPR/Cas9 dual knockout with MpE(z)3 in females, profiled by transcriptome and CUT&RUN. Maternal inheritance is essential for paternal H3K27me3 deposition. Loss reduces H3K27me3, reactivates paternal genes, drops embryo survival to ~20% and abolishes viable spores.
Mp5g22900	MpE(z)3	experimental subject	PRC2 catalytic subunit; CRISPR/Cas9 dual knockout with MpE(z)2 in females, profiled by transcriptome and CUT&RUN. Maternal copy required for paternal chromosome H3K27me3; combined loss impairs imprinting, reactivates paternal alleles and causes embryonic lethality.
—	MpEz2 missing	experimental subject	E(z)/EZH Polycomb subunit; targeted (CRISPR) in paternal-chromosome H3K27me3 repression.
—	MpEz3 missing	experimental subject	E(z)/EZH Polycomb subunit; CRISPR target in Polycomb-mediated repression.
Mp5g18040	MpE(z)1	experimental comparator	Expressed in all tissues including embryos; not functionally knocked out due to haploid lethality, so likely accounts for residual H3K27me3 in e(z)2/e(z)3 mutants, indicating redundancy among PRC2 catalytic subunits.
Mp3g06080	MpFIE	experimental background	Non-catalytic PRC2 subunit; expression profiling only, expressed across developmental stages, supporting a functional Polycomb complex in Marchantia.
—	MpMSI1 missing	experimental background	Non-catalytic PRC2 subunit; expression profiling only, constitutively expressed, completing the Marchantia PRC2 complex composition.
—	MpSu(z)12 missing	experimental background	Non-catalytic PRC2 subunit; expression profiling only, constitutively expressed, contributing to PRC2 subunit composition.