Rong, D., et al. · 2022 · PNAS research
doi:10.1073/pnas.2117803119 PMID:36375069 PMC9704705
| Gene ID | Name | Evidence / Role | Function (this paper) |
|---|---|---|---|
| Mp7g17540 | MpROP | experimental subject | Sole ROP GTPase in Marchantia; CRISPR knockouts (Mprop-1/-2) show retarded rhizoid elongation and disorganized cell division. Regulates spatial cell-division patterns and meristem notch specification, localizing polarly to the plasma membrane. AtROP2 and complementation rescue wild-type phenotypes. |
| — | MpROPGEF missing | experimental subject | Encoded by the karappo (kar) gene; upstream activator of MpROP. Interacts with MpROP by yeast two-hybrid, and kar mutants phenocopy gemma defects; the Mprop-1 switch II mutation blocks MpROPGEF binding. |
| Mp1g12750 | MpARF1 | experimental comparator | AUXIN RESPONSE FACTOR1; Mparf1 knockout shows variable meristem-notch numbers, used as a notch-patterning comparator. |
| Mp3g23400 | MpEF1 | experimental tool | Constitutive promoter used to drive transgene expression in complementation experiments. |
| Mp8g09680 | MpREN | citation background | RhoGAP ortholog cited from prior work; knockout reportedly causes rhizoid growth defects, supporting ROP pathway context. |
| — | MpSPK1 missing | sequence background | DOCK-family GEF homolog identified in the genome; candidate MpROP activator but no yeast two-hybrid interaction detected. |
| — | MpSWAP70 missing | sequence background | Conserved Rho GEF homolog; speculative alternative MpROP activator pending further study. |
| Mp8g08780 | MpYUC2 | experimental background | Auxin biosynthesis gene highly expressed at meristem notches; expression decreases in Mprop mutants, linking ROP to auxin. |