Aki, S. S., et al. · 2022 · Scientific Reports research
doi:10.1038/s41598-022-25684-3 PMID:36477255 PMC9729187
| Gene ID | Name | Evidence / Role | Function (this paper) |
|---|---|---|---|
| Mp6g04830 | MpGCAM1 | experimental subject | R2R3-MYB transcription factor functioning downstream of cytokinin signaling; knockout mutants show defective gemma cup formation while inducible overexpression yields undifferentiated cell clumps. Indirectly upregulated by MpRRB-mediated signaling (not a direct target) and requires de novo protein synthesis for induction. |
| Mp3g03810 | MpRRA | experimental subject | Type-A response regulator acting as a negative regulator of cytokinin signaling; knockout enhances cytokinin signaling and increases gemma cup formation. Cytokinin-induced in wild type but not in Mprrb, and the Mpgcam1 Mprra double mutant produces curled thalli without gemma cups. |
| Mp4g20600 | MpRRB | experimental subject | Type-B response regulator central to cytokinin signaling; knockout lines show defective gemma cup formation and increased rhizoids. Indirectly upregulates MpGCAM1 and drives expression of nitrogen-transport and auxin-related genes; active at the apical notch of young thalli. |
| Mp5g10910 | MpCKX2 | experimental tool | Cytokinin oxidase whose overexpression reduces endogenous cytokinins, used to lower cytokinin levels and assay MpGCAM1 expression (proMpGCAM1:GUS); overexpressing lines show fewer/no gemmiparous cells and reduced MpGCAM1 transcripts. |
| Mp3g23400 | MpEF1 | experimental tool | Elongation factor 1-alpha used as the constitutive reference gene to normalize mRNA levels in qRT-PCR analyses. |
| Mp5g03090 | MpCKX1 | sequence background | One of two cytokinin oxidase genes in M. polymorpha; listed among upregulated genes in RNA-seq but not functionally characterized here. |
| Mp2g14010 | MpGH3B | experimental background | Acyl acid amido synthetase gene downregulated by MpRRB-mediated cytokinin signaling, indicating adjustment of auxin homeostasis. |
| Mp1g16480 | MpIPT1 | sequence background | One of two cytokinin biosynthesis genes; not upregulated by MpGCAM1 overexpression, mentioned as background to cytokinin biosynthesis. |
| Mp8g02960 | MpIPT2 | sequence background | Cytokinin biosynthesis gene orthologous to Arabidopsis IPT9; downregulated under MpRRB-mediated signaling and not upregulated by MpGCAM1 overexpression. |
| Mp6g07950 | MpPIN3 | experimental background | Auxin efflux carrier gene induced by MpRRB-mediated cytokinin signaling, cited as evidence of cytokinin-auxin cross-talk. |