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Comprehensive analysis of peptide-coding genes and initial characterization of an LRR-only microprotein in Marchantia polymorpha.

Kobayashi, H., et al. · 2023 · Frontiers in Plant Science   research

doi:10.3389/fpls.2022.1051017   PMID:36756228   PMC9901580

Linked genes (33)  7 core 26 peripheral

Gene ID Name Evidence / Role Function (this paper)
Mp3g10170 MpSGF10B missing experimental subject Main subject of the paper: a secreted LRR-only microprotein with an N-terminal signal peptide and two LRR domains. Expressed in apical notches, gemma cup bottoms, and antheridia; YFP fusion localizes to the cell periphery and is secreted. CRISPR/Cas9 mutants (Mpsgf10b-1ge, -2ge) showed slightly increased antheridiophore number.
MpSGF13A missing experimental subject Characterized candidate peptide gene: RT-PCR-detected expression with intense GUS activity in thalli and antheridiophores; YFP fusion localized to cytosolic compartments.
MpSGF13B missing experimental subject Characterized candidate peptide gene: intense GUS reporter activity in thalli and antheridiophores; YFP fusion showed cytosolic localization.
MpSGF13C missing experimental subject Characterized candidate peptide gene of the SGF13 group: no observable GUS activity; YFP fusion localized to cytosolic compartments.
Mp3g15380 MpSGF17 missing experimental subject Characterized candidate peptide gene: expressed in antheridia and archegonia by GUS reporter, with chloroplast co-localization of the YFP fusion in transient assays.
MpSGF18A missing experimental subject Characterized candidate peptide gene: antheridiophore-specific GUS activity and cytosolic localization of the YFP fusion in transient expression.
Mp2g23830 MpSGF6 missing experimental subject One of the candidate peptide genes characterized: RT-PCR detected expression in thalli and antheridiophores, GUS reporter showed antheridiophore-specific activity, and YFP fusion co-localized with chloroplasts.
Mp3g23300 MpBNB citation tool Cited as a marker gene for antheridiophore/reproductive development.
MpEXP missing experimental tool Auxin-responsive gene used as a readout; its expression was unaltered in Mpsgf10bge mutants.
Mp1g09500 MpWIP experimental tool Auxin-responsive gene used as a readout; its expression was unaltered in Mpsgf10bge mutants.
Mp1g07140 missing sequence background Locus ID for MpSGF1, listed in the candidate peptide gene table.
Mp3g15410 missing sequence background Locus ID for MpSGF12, listed in the candidate peptide gene table.
Mp4g08500 missing sequence background Locus ID for MpSGF8, listed in the candidate peptide gene table.
Mp4g19140 missing sequence background Locus ID for MpSGF11A, listed in the candidate peptide gene table.
Mp5g08160 missing sequence background Locus ID for MpSGF18A, listed in the candidate peptide gene table.
Mp6g01390 missing sequence background Locus ID for MpSGF2, listed in the candidate peptide gene table.
Mp6g09670 missing sequence background Locus ID for MpSGF14, listed in the candidate peptide gene table.
Mp7g00660 missing sequence background Locus ID for MpSGF13A, listed in the candidate peptide gene table.
Mp7g00670 missing sequence background Locus ID for MpSGF13B, listed in the candidate peptide gene table.
Mp7g02600 missing sequence background Locus ID for MpSGF4, listed in the candidate peptide gene table.
Mp7g14620 missing sequence background Locus ID for MpSGF13C, listed in the candidate peptide gene table.
Mp8g00200 missing sequence background Locus ID for MpSGF7, listed in the candidate peptide gene table.
missing sequence background Locus ID for MpSGF15, listed in the candidate peptide gene table.
Mp3g07430 MpDIR2 sequence background Locus ID for MpSGF16F, listed in the candidate peptide gene table.
Mp4g14630 MpDIR23 sequence background Locus ID for MpSGF16A, listed in the candidate peptide gene table.
Mp5g00590 MpDIR27 sequence background Locus ID for MpSGF16D, listed in the candidate peptide gene table.
Mp7g13800 MpDIR3 sequence background Locus ID for MpSGF16B, listed in the candidate peptide gene table.
Mp3g23830 MpDIR49 sequence background Locus ID for MpSGF6, listed in the candidate peptide gene table.
Mp8g16530 MpDIR56 sequence background Locus ID for MpSGF16E, listed in the candidate peptide gene table.
MpPP1I5 sequence background Locus ID for MpSGF3, listed in the candidate peptide gene table.
MpSGF18B missing experimental background Listed in the candidate gene table (SGF18 group, Mp4g23800); not experimentally characterized.
Mp4g19080 MpUGT21 sequence background Locus ID for MpSGF11B, listed in the candidate peptide gene table.
MpVg01020 missing sequence background Locus ID for MpSGF9, listed in the candidate peptide gene table.