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An enhancer trap system to track developmental dynamics in Marchantia polymorpha.

Marron, A. O., et al. · 2023 · The Plant Journal   research

doi:10.1111/tpj.16394   PMID:37583263   PMC10952768

Linked genes (11)  0 core 11 peripheral

Gene ID Name Evidence / Role Function (this paper)
Mp4g00190 missing sequence subject Candidate gene near the ET239-P54 insertion site, encoding a small protein of unknown function with no known homology to other plant genes.
Mp4g04240 missing sequence subject Candidate gene near the insertion site of the apical notch marker line ET239-P161. BLAST shows similarity to an embryophyte-specific gene family found across all land plants.
Mp4g21310 missing sequence subject Candidate gene near the ET239-P64 margin marker insertion site, encoding a protein of unknown function, identified by genomic analysis.
Mp4g21320 missing sequence subject Candidate gene near the ET239-P64 margin marker insertion site, annotated as a cyclophilin-type peptidyl-prolyl isomerase, identified by genomic analysis.
Mp4g21300 Mp1R-MYB17 sequence subject Candidate gene near the insertion site of the margin tissue marker line ET239-P64. Noted from RNAseq to be strongly up-regulated by auxin (2,4-D) treatment.
Mp3g17930 MpRSL1 sequence subject Identified by genomic analysis as the candidate gene near the insertion site of the rhizoid marker line ET239-P177. Annotated as the Marchantia homolog of Root Hairless-like 6 (RSL6), a transcription factor implicated in rhizoid/root-hair development.
Mp4g00200 MpWRKY12 sequence subject Candidate gene near the insertion site of the oil cell marker line ET239-P54, found enriched in the oil cell cluster. A WRKY-family transcription factor consistent with oil cells' role in anti-herbivore defense.
Mp4g00180 MpWRKY13 sequence subject WRKY-family candidate gene located near the ET239-P54 oil cell marker insertion site, identified via genomic analysis of the trapped region.
Mp3g21660 MpPIN1 experimental tool Used as a marker line to visualize polar auxin transport; elevated auxin treatment increased the spatial distribution of MpPIN1 expression as predicted.
Mp8g08780 MpYUC2 experimental tool Used as a validation marker line; auxin and inhibitor treatments confirmed the expected response, demonstrating treatments did not non-specifically alter enhancer trap signals.
Mp5g06970 MpERF20 citation background Cited from prior work: over-expression of MpLAXR (MpERF20) caused strong cell division at gemma edges, discussed as context for margin tissue regulation.