Sun, R., et al. · 2023 · Plant Cell research
doi:10.1093/plcell/koad216 PMID:37597168 PMC10615216
| Gene ID | Name | Evidence / Role | Function (this paper) |
|---|---|---|---|
| — | MpDTPS3 missing | experimental subject | Diterpene synthase 3 (= MpCPS); ent-copalyl diphosphate synthase in GA-related biosynthesis. |
| — | MpDTPS4 missing | experimental subject | Diterpene synthase 4 (= MpKS); kaurene synthase-type in GA-related biosynthesis. |
| Mp4g23680 | MpKAOL1 | experimental subject | Loss-of-function mutant (Mp_kaol1ge) generated and assayed in yeast. Yeast expressing MpKAOL1 consumed KA and produced GA12, identifying it as the active KAO downstream of KA in GAMp biosynthesis. |
| Mp1g25410 | MpKAOL2 | experimental subject | KAO-like cytochrome P450 (Mp1g25410); gibberellin-related diterpenoid biosynthesis modulating far-red responses. |
| Mp3g18320 | MpKOL1 | experimental subject | Loss-of-function mutant Mp_kol1ld generated and enzyme assayed in yeast. Yeast expressing MpKOL1 consumed ent-kaurenoic acid (KA) and produced GA12; mutant showed enlarged thalli under cW+cFR, identifying MpKOL1 as the active KO required for FR response. |
| Mp3g17350 | MpPIF | experimental subject | Knockout Mp_pifko analyzed by transcriptome; encodes a PIF transcription factor. FR-induced up-regulation of biosynthesis genes (e.g. MpKOL1) occurred only in wild-type and not Mp_pifko, showing MpPIF mediates FR responses. |
| Mp6g05430 | MpTPS1 | experimental subject | Loss-of-function mutant Mp_tps1ld generated; minor ent-kaurene producer. Mutant morphology was similar to wild-type under cW and cW+cFR, indicating MpTPS1 is not a major gene required for GAMp biosynthesis. |
| Mp2g07200 | MpTPS3 | experimental subject | CRISPR large-deletion mutants (Mp_cpsld) were generated; encodes the first enzyme of GA-related diterpenoid biosynthesis. Under cW+cFR, mutant thalli were significantly larger than wild-type, showing MpCPS is required for far-red-light developmental responses (hyponasty, slender thallus). |
| Mp6g05950 | MpTPS4 | experimental subject | Loss-of-function mutant Mp_ksld was generated; acts downstream of MpCPS producing ent-kaurene. Mutant phenotype under cW+cFR resembled Mp_cpsld with significantly larger thalli, indicating MpKS is required for the FR response. |
| Mp2g10420 | MpKAOL3 | experimental comparator | Homolog whose complete-deletion mutant Mp_kaol3ld produced no GA12 and showed no severe FR-response defect, lacking MpKAOL1's activity. |
| Mp2g01950 | MpKOL2 | experimental comparator | Homolog tested in yeast; produced no KA and its mutant showed no FR-response phenotype, so it lacks the KA-producing activity of MpKOL1. |
| Mp2g01940 | MpKOL3 | experimental comparator | Homolog tested in yeast; produced no KA and its mutant showed no FR-response phenotype, lacking MpKOL1's activity. |
| Mp3g23300 | MpBNB | experimental tool | Citrine knock-in line (MpBNB-Cit) used as a marker; MpBONOBO protein accumulated in initial cells and immature gametangia to visualize early gametangium differentiation. |
| Mp5g19490 | MpABA4 | experimental background | DEG: ABA metabolic gene induced by FR enrichment. |
| Mp5g10910 | MpCKX2 | experimental background | DEG: cytokinin deactivation gene (CYTOKININ OXIDASE2) down-regulated in Mp_cps-4ld. |
| Mp2g25940 | MpCYP707A | experimental background | DEG: ABA metabolic gene altered by FR enrichment. |
| Mp5g20660 | MpGRAS6 | citation background | Cited as a GA-signaling repressor; its overexpression inhibits MpPIF-mediated FR responses, referenced to distinguish GAMp signaling. |
| Mp8g02960 | MpIPT2 | experimental background | DEG: cytokinin biosynthesis gene (ISOPENTENYLTRANSFERASE2) down-regulated in Mp_cps-4ld. |
| Mp1g00270 | MpLOG | experimental background | DEG: cytokinin-activating gene (LONELY GUY) down-regulated in Mp_cps-4ld. |
| Mp2g07800 | MpNCED | experimental background | DEG: ABA biosynthesis gene induced by FR enrichment. |
| Mp3g13150 | MpTPS6 | experimental background | DEG: expression encoding a cis-kolavenol-producing enzyme was significantly decreased in Mp_cps-4ld. |