Yamamoto, C., et al. · 2024 · Proceeding of the National Academy of Sciences USA research
doi:10.1073/pnas.2322211121 PMID:38593080 PMC11032487
| Gene ID | Name | Evidence / Role | Function (this paper) |
|---|---|---|---|
| Mp7g08500 | MpCAPE | experimental subject | Dual adenylyl cyclase/phosphodiesterase that synthesizes and hydrolyzes cAMP, expressed during maturation of male reproductive organs. Knockout mutants are male-infertile with impaired flagellar motility, swimming in circles instead of straight (16.4 vs 43.5 um/s WT). Complementation restored normal motility, identifying it as the central cAMP regulator of sperm motility. |
| Mp2g09970 | MpPKAR1 | experimental subject | PKA regulatory subunit acting as a downstream cAMP effector, highly expressed in antheridia. Double-knockout with MpPKAR2 caused abnormal/trembling sperm motility and reduced swimming linearity and speed comparable to CAPE mutants. Complementation of MpPKAR1 restored normal motility. |
| Mp8g17350 | MpPKAR2 | experimental subject | PKA regulatory subunit and cAMP effector, highly expressed in antheridia. MpPKAR1/MpPKAR2 double-knockout mutants showed trembling/immotile sperm with defects in swimming linearity and speed, demonstrating PKA function downstream of cAMP in sperm motility. |
| Mp3g23400 | MpEF1 | citation tool | Elongation factor used as an internal reference for normalizing transcript levels in expression heat maps. |
| — | MpPKAC | sequence background | PKA catalytic subunit identified by CNB domain search; conserved PKA-C inhibitor-site signature aligning with mammalian PKA-Calpha/beta, not experimentally tested. |
| Mp6g02750 | MpTIR1 | citation background | Auxin receptor ortholog cited as an alternative cAMP source but ruled out, since it acts in vegetative tissues not male organs. |