Mp7g08500



Description

Annotation

Database ID Description
MobiDBLite mobidb-lite consensus disorder prediction
Coils Coil Coil
SUPERFAMILY SSF55073 Nucleotide cyclase
CDD cd07302 CHD
SUPERFAMILY SSF109604 HD-domain/PDEase-like
CDD cd00077 HDc
ProSitePatterns PS00126 3'5'-cyclic nucleotide phosphodiesterase domain signature.
Gene3D G3DSA:3.30.70.1230 Nucleotide cyclase
Pfam PF00211 Adenylate and Guanylate cyclase catalytic domain
PRINTS PR00387 3'5'-cyclic nucleotide phosphodiesterase signature
Gene3D G3DSA:1.10.1300.10 -
SMART SM00044 cyc_6
Pfam PF00233 3'5'-cyclic nucleotide phosphodiesterase
ProSiteProfiles PS50125 Guanylate cyclase domain profile.
ProSiteProfiles PS51845 3'5'-cyclic nucleotide phosphodiesterase domain profile.
SMART SM00471 hd_13
PANTHER PTHR43336 OXYGEN SENSOR HISTIDINE KINASE RESPONSE REGULATOR DEVS/DOSS
KOG KOG3689 Cyclic nucleotide phosphodiesterase; N-term missing; [T]
KOG KOG2133 Transcriptional corepressor Atrophin-1/DRPLA; C-term missing; [R]
MapolyID Mapoly0068s0004 -
GO GO:0009190 cyclic nucleotide biosynthetic process
GO GO:0008081 phosphoric diester hydrolase activity
GO GO:0035556 intracellular signal transduction
GO GO:0004114 3',5'-cyclic-nucleotide phosphodiesterase activity
GO GO:0007165 signal transduction

Nomenclature

Gene symbol Product Transcript ID Status
MpCAPE adenylyl cyclase with a phosphodiestrase domain Published

Associated Literature (AI-based literature mining, Beta-version)

Cloned from Marchantia as a fusion protein with an N-terminal cyclic-nucleotide phosphodiesterase domain and a C-terminal class III adenylyl cyclase domain; recombinant GST-MpCAPE-AC produced cAMP (Mn2+ enhancing activity ~35-fold over Mg2+) and complemented an E. coli AC-deficient mutant, while His-MpCAPE-PDE hydrolyzed cAMP/cGMP, with catalytic residues (Asp-1340 for AC; His-199/203 for PDE) confirmed essential by mutagenesis. GUS reporter showed expression in the antheridium, implicating it in male reproductive development.

4 core 1 peripheral

Yamamoto, C., et al. (2024) · Proceeding of the National Academy of Sciences USA  research experimental subject
Dual adenylyl cyclase/phosphodiesterase that synthesizes and hydrolyzes cAMP, expressed during maturation of male reproductive organs. Knockout mutants are male-infertile with impaired flagellar motility, swimming in circles instead of straight (16.4 vs 43.5 um/s WT). Complementation restored normal motility, identifying it as the central cAMP regulator of sperm motility.
Hayashida, Y., et al. (2021) · Journal of Plant Research  research experimental subject
Bifunctional adenylyl cyclase / cAMP phosphodiesterase (CAPE); its PDE domain (MpCAPE-PDE) was purified from E. coli and kinetically characterized (Km/Vmax), establishing cAMP-hydrolyzing activity alongside its AC activity.
Yamamoto, C., et al. (2021) · Scientific Reports  research experimental subject
Marchantia CAPE encoding combined class III adenylyl cyclase (C-terminal) and class I phosphodiesterase (N-terminal) domains; biochemical assays confirmed both domains are enzymatically active, with cAMP-specific PDE and a low-activity high-affinity AC. Shown to be specifically expressed in spermatogenous cells of antheridia but not vegetative organs, serving as the functional reference for CAPE in motile-sperm streptophytes.
Kasahara, M., et al. (2016) · Scientific Reports  research experimental subject
Cloned from Marchantia as a fusion protein with an N-terminal cyclic-nucleotide phosphodiesterase domain and a C-terminal class III adenylyl cyclase domain; recombinant GST-MpCAPE-AC produced cAMP (Mn2+ enhancing activity ~35-fold over Mg2+) and complemented an E. coli AC-deficient mutant, while His-MpCAPE-PDE hydrolyzed cAMP/cGMP, with catalytic residues (Asp-1340 for AC; His-199/203 for PDE) confirmed essential by mutagenesis. GUS reporter showed expression in the antheridium, implicating it in male reproductive development.
Ren, X., et al. (2024) · Plant Cell  research experimental tool
Antheridium-expressed gene used as a marker; activated in MpGLID-overexpressing transgenics.

Expression Level powered by MBEX

Link to the original image in MBEX

Transcript models

Transcript ID Location Sequences Extract region
Mp7g08500.1 chr7:11489973..11502337 (-) Gene /  mRNA /  CDS /  Protein
FASTA / GenBank (with flanking bases)

Gene structure:

Sequences:

Gene UTR + CDS + intron

FASTA

mRNA UTR + CDS

FASTA

CDS

FASTA

Protein

FASTA


MarpolBase / Genome Informatics Lab. NIG.