Ren, X., et al. · 2024 · Plant Cell research
doi:10.1093/plcell/koae206 PMID:39041486 PMC11371191
| Gene ID | Name | Evidence / Role | Function (this paper) |
|---|---|---|---|
| Mp3g23300 | MpBNB | experimental subject | bHLH transcription factor (Mp3g23300) acting upstream of MpGLID to dictate germline fate entry; overexpression induces male germ cell-like cells. Y1H and EMSA show MpBNB directly binds and activates the MpGLID promoter. |
| Mp3g17000 | MpGLID | experimental subject | PHD finger protein (Mp3g17000) preferentially expressed early in antheridia; loss-of-function fails to specify sperm cells and overexpression induces ectopic male germline cells. Acts as a master regulator of both male and female germline specification, directly activated by MpBNB via an E-box (CACGTG) in its promoter. |
| Mp3g06700 | missing | experimental comparator | Antheridium-specific PHD subclade II gene peaking at late stage; loss-of-function mutants showed no effect on male gametogenesis, contrasting MpGLID. |
| Mp7g08500 | MpCAPE | experimental tool | Antheridium-expressed gene used as a marker; activated in MpGLID-overexpressing transgenics. |
| Mp5g07890 | MpDEL2 | experimental tool | Antheridium-expressed gene used as a marker; activated in MpGLID-overexpressing transgenics. |
| Mp3g23400 | MpEF1 | experimental tool | MpEF1alpha promoter used as the constitutive promoter for transgenic overexpression constructs. |
| Mp2g23000 | MpBZR3 | experimental background | BZR/BES TF gene, an early antheridium-specific marker essential for early antheridium development and upregulated in MpGLID-overexpressing plants. |
| Mp2g20695 | MpLRL | citation background | Cited alongside CKI1 as securing specific, stable expression of MpBNB. |