Hernández-García, J., et al. · 2024b · Proceeding of the National Academy of Sciences USA research
doi:10.1073/pnas.2319163121 PMID:38696472 PMC11087773
| Gene ID | Name | Evidence / Role | Function (this paper) |
|---|---|---|---|
| Mp5g20660 | MpGRAS6 | experimental subject | Tested by yeast two-hybrid, BiFC, and dual-luciferase assays; its N-terminal domain bound the MpMED15 KIX domain and its GRAS domain bound MpMYB14. Acts as a transcriptional coactivator, enhancing MpMYB14-driven MpPAL activation, demonstrating conserved DELLA-MED15 coactivation in land plants. |
| Mp8g02180 | MpMED15 missing | experimental subject | Identified as the single full-length MED15 gene in M. polymorpha; its KIX domain interacted with MpDELLA's N-terminus by Y2H/BiFC and coexpression in luciferase assays further boosted MpMYB14/MpDELLA-dependent MpPAL activation, supporting conserved Mediator recruitment. |
| Mp5g19050 | MpR2R3-MYB14 | experimental subject | Shown by Y2H and BiFC to interact with MpDELLA via its GRAS domain; in dual-luciferase assays it activated the MpPAL promoter, with this activity enhanced by MpDELLA and further by MpMED15 in a gene-specific manner. |
| Mp1g05190 | MpPAL paper
MpPAL10 nomenclature |
experimental tool | Its promoter was used as a luciferase reporter target to assay MpMYB14/MpDELLA/MpMED15 coactivation. |