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D4-dn-iso-OPDA, a bioactive plant hormone of Marchantia polymorpha.

Kaji, T., et al. · 2024 · iScience   research

doi:10.1016/j.isci.2024.110191   PMID:38974968   PMC11225365

Linked genes (6)  2 core 4 peripheral

Gene ID Name Evidence / Role Function (this paper)
Mp2g26590 MpCOI1 experimental subject The jasmonate receptor F-box protein. This paper shows Δ4-dn-iso-OPDA activates jasmonate responses in an MpCOI1-dependent manner, tested with the Mpcoi1-2 mutant. Pull-down assays and docking confirmed ligand binding to the MpCOI1-MpJAZ co-receptor.
Mp6g06230 MpJAZ experimental subject The co-receptor/repressor partner of MpCOI1. Binding assays and structural docking demonstrated that Δ4-dn-iso-OPDA promotes MpCOI1-MpJAZ co-receptor complex formation, supporting its role as the bioactive ligand.
Mp5g16920 MpDIR33 experimental tool Dirigent-like jasmonate-signaling marker gene measured by RT-qPCR to read out hormone-induced responses.
Mp5g21220 MpPAT experimental tool Patatin-like jasmonate-signaling marker gene measured by RT-qPCR to read out hormone-induced responses.
Mp7g06220 MpDIR17 citation background Allene oxide cyclase, a conserved enzyme of the upstream OPDA biosynthesis pathway referenced as pathway context, not investigated in this study.
Mp3g10660 MpFAD5 citation background Fatty-acid desaturase required for PUFA/dn-OPDA biosynthesis; cited from prior work showing dn-cis/iso-OPDA accumulation is abolished in the Mpfad5-3 mutant.