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Inositol pyrophosphate catabolism by three families of phosphatases regulates plant growth and development.

Laurent, F., et al. · 2025 · PLoS Genetics   research

doi:10.1371/journal.pgen.1011468   PMID:39531477   PMC11611267

Linked genes (8)  3 core 5 peripheral

Gene ID Name Evidence / Role Function (this paper)
Mp5g06600 MpDDP1 experimental subject Characterized as an inositol 1-pyrophosphate phosphatase cleaving 1-InsP7, clustering with AtNUDT17/18/21 and yeast Ddp1. Mutants show only mild growth defects with slightly reduced thallus surface and elevated 1-InsP7 and 1,5-InsP8; double mutants with MpPFA-DSP1 are not viable.
Mp3g10950 MpPFA-DSP1 missing experimental subject Characterized as a specific inositol 5-pyrophosphate phosphatase preferring 5-InsP7 over 1,5-InsP8, clustering phylogenetically with Arabidopsis PFA-DSPs and yeast Siw14. Loss-of-function mutants show vertical thallus growth, reduced surface area, more rhizoids, fewer gemma cups, elevated 5-InsP7, and altered cell wall and PSI gene expression.
Mp8g06840 MpVIH experimental subject Single PPIP5K gene encoding a bifunctional kinase/phosphatase. Mutants resemble Mppfa-dsp1 with vertical growth, reduced thallus, more rhizoids, fewer gemma cups, lower 1,5-InsP8 and higher 5-InsP7; the Mppfa-dsp1 Mpvip1 double mutant is severely stunted with high nitrate.
Mp4g19710 MpPHO1;H4 missing experimental tool Used as a PSI marker gene; decreased in Mppfa-dsp1ge/Mpvip1ge and increased in Mpnudt1ge mutants.
Mp2g20620 MpPHT1;4 missing experimental tool Pi transporter used as a PSI marker gene; elevated in Mppfa-dsp1ge and Mpvip1ge mutants.
Mp1g27550 MpSPX experimental tool Used as a phosphate-starvation-induced (PSI) marker gene; its transcript decreased in Mppfa-dsp1ge and Mpvip1ge mutants.
Mp1g22660 MpIPMK sequence background A PP-InsP biosynthetic enzyme whose transcript was quantified by RNA-seq; no loss-of-function mutant generated.
Mp2g24850 MpITPK1 sequence background A PP-InsP biosynthetic enzyme whose transcript was quantified by RNA-seq; no loss-of-function mutant generated.