Rienstra, J., et al. · 2025 · Proceeding of the National Academy of Sciences USA research
doi:10.1073/pnas.2501915122 PMID:40168121 PMC12002309
| Gene ID | Name | Evidence / Role | Function (this paper) |
|---|---|---|---|
| Mp1g12750 | MpARF1 | experimental subject | Functionally studied as the sole A-class positive effector of auxin transcription: alanine substitutions (R194, P197, R199) and B3-domain swaps were assayed for complementation of the Mparf1-4 null. RNA-seq showed Tak-1 responded to IAA (112 DEGs) while Mparf1 showed no auxin response, defining R194/P197/R199 as essential for its DNA-binding function. |
| Mp1g07070 | MpARF3 | experimental subject | C-class ARF assessed by complementation: its DBD failed and its B3 domain only partially complemented Mparf1-4, indicating C-class DNA-binding specificity diverged early; B3 is entirely conserved across accessions. |
| Mp4g11820 | MpARF2 | experimental comparator | B-class ARF whose B3 domain was used for structural/docking comparison and swapped into MpARF1, fully complementing the Mparf1 mutant (functionally similar B3). |
| — | MpAPT3 missing | experimental tool | Reference gene used for RT-qPCR normalization (primers JR163/JR164). |