Futagami K, Tsuzuki M, Yoshida M, Watanabe Y. · 2025 · Journal of experimental botany research
doi:10.1093/jxb/eraf148 PMID:40200901 PMC12369473
| Gene ID | Name | Evidence / Role | Function (this paper) |
|---|---|---|---|
| — | MpmiR319 missing | experimental subject | The mature miRNA encoded by the two MIR319 loci. The paper shows MpmiR319 promotes gemma/gemma cup formation by targeting and suppressing MpRKD and MpR2R3-MYB21 transcripts; loss of miR319 reduced gemma cup number. |
| Mp3g08965 | MpMIR319A missing | experimental subject | One of the two M. polymorpha MIR319 loci producing the mature miR319. CRISPR knockout of MpMIR319A (with MpMIR319B) reduced gemma cup formation, demonstrating its role in promoting gemma/gemma cup development. |
| Mp4g01845 | MpMIR319B missing | experimental subject | The second M. polymorpha MIR319 locus producing the same mature miR319; mir319b alleles (e.g. mir319bge-4) showed severe inhibition of gemma cup formation, supporting a role in promoting gemma/gemma cup development. |
| Mp3g05910 | MpR2R3-MYB21 | experimental subject | GAMYB-family R2R3-MYB transcription factor identified as a second MpmiR319 target; miR319-resistant MpR2R3-MYB21 did not yield the gemma-cup-less phenotype, indicating it is less crucial than MpRKD for gemma cup formation. |
| Mp3g04030 | MpRKD | experimental subject | RWP-RK domain-containing transcription factor identified as a target of MpmiR319, expressed in the rim and bottom of gemma cups. Expression of a miR319-resistant MpRKD produced gemma/gemma cup-less plants, indicating its repression by miR319 is critical for proper gemma cup formation. |
| — | MpKAI2 missing | citation background | Cited as one of the known pathways mediating gemma cup formation in M. polymorpha. |