Melissa DÁ, Luis LJ, Eduardo FS, Annie EC, Domingo MÁ, Tom FJ, Jesús LR, Fernando OM, John BL, Mario AA, Cruz-Ramírez A. · 2025 · Physiologia plantarum research
doi:10.1111/ppl.70365 PMID:40583831 PMC12207571
| Gene ID | Name | Evidence / Role | Function (this paper) |
|---|---|---|---|
| Mp6g08530 | missing | experimental subject | One of three M. polymorpha AGC kinases (closest to AtPINOID) implicated in MpPIN1 phosphorylation; expression examined by RT-qPCR. |
| Mp8g11302 | missing | experimental subject | One of three M. polymorpha AGC kinases (closest to AtPINOID) implicated in MpPIN1 phosphorylation; expression examined by RT-qPCR. |
| Mp8g16840 | missing | experimental subject | One of three M. polymorpha AGC kinases (closest to AtPINOID) implicated in MpPIN1 phosphorylation; expression examined by RT-qPCR. |
| Mp8g11450 | MpAP2L1 | experimental subject | The sole AINTEGUMENTA-LIKE/PLT/BBM ortholog in M. polymorpha and the central subject. Using transcriptional fusions, auxin (2,4-D/NPA) treatments, 35S:MpANT-CITRINE overexpression, CRISPR loss-of-function, RT-qPCR, immunolocalization and EdU assays, MpANT was shown to be auxin-induced and meristem-enriched; loss-of-function reduced plant size, meristematic activity and rhizoids while overexpression expanded proliferative areas. MpANT acts upstream of MpPIN1, forming an auxin-MpANT-MpPIN1 feedforward loop. |
| Mp1g12750 | MpARF1 | experimental subject | Auxin response factor in the MpANT co-expression cluster; moderately downregulated in MpANT loss-of-function by RT-qPCR. |
| Mp4g11820 | MpARF2 | experimental subject | Auxin response factor co-expressed with MpANT and assessed in the RT-qPCR expression analysis of MpANT mutants. |
| Mp1g07070 | MpARF3 | experimental subject | Auxin response factor in the MpANT co-expression cluster; moderately downregulated in MpANT loss-of-function by RT-qPCR. |
| — | MpCYCD missing | experimental subject | D-type cyclin associated with cell proliferation; expression examined by RT-qPCR as a candidate MpANT-regulated proliferation gene. |
| Mp1g10440 | MpGRAS3 | experimental subject | GRAS-family gene linked to cell proliferation/meristem maintenance; a putative MpANT target whose expression was examined by RT-qPCR in MpANT mutants. |
| Mp3g21660 | MpPIN1 | experimental subject | Auxin-efflux transporter analyzed as a major MpANT target. RT-qPCR and PIN1 immunolocalization showed MpANT modulates MpPIN1 expression/localization and auxin distribution; an APB-binding site is present in the MpPIN1 promoter. |
| Mp5g05140 | MpPIN2 | experimental subject | Auxin-efflux transporter whose expression was examined by RT-qPCR in MpANT mutants. |
| Mp5g14320 | MpTAA | experimental subject | Auxin-biosynthesis (TAA) gene; a putative MpANT target carrying an APB-binding site in its promoter and moderately downregulated in MpANT loss-of-function by RT-qPCR. |
| Mp5g07790 | MpYUC1 | experimental subject | Auxin-biosynthesis (YUCCA) gene examined by RT-qPCR; moderately downregulated in MpANT loss-of-function mutants. |
| Mp8g08780 | MpYUC2 | experimental subject | Auxin-biosynthesis (YUCCA) gene co-expressed with MpANT and examined by RT-qPCR; moderately downregulated in MpANT loss-of-function. |
| — | MpACT7 missing | experimental tool | Used as the internal reference gene for RT-qPCR normalization. |
| Mp8g09250 | MpASLBD17 | citation background | Co-expressed with MpANT in the young sporophyte region. |
| Mp4g12420 | MpBHLH44 | citation background | Co-expressed with MpANT in antheridia/archegonia regions. |
| Mp2g03050 | MpCHK1 | citation background | Co-expressed with MpANT in antheridia/archegonia regions. |
| Mp6g07050 | MpCLE1 | citation background | Cited from Liu et al. as a transcriptional target through which MpANT restricts cell proliferation. |
| Mp1g02840 | MpDCL3 paper
MpDCL1a nomenclature |
citation background | Co-expressed with MpANT in antheridia/archegonia regions. |
| Mp5g21990 | MpGRAS9 | citation background | Cited from Liu et al. as a direct MpANT target. |
| Mp8g16900 | MpGRF | citation background | Growth-regulating factor co-expressed with MpANT in apical cells. |
| Mp6g05000 | MpIAA | citation background | Aux/IAA auxin-signaling gene mentioned in the MpANT co-expression cluster. |
| Mp1g02860 | MpLFY | citation background | Co-expressed with MpANT in antheridia/archegonia regions. |
| Mp4g11760 | MpMADS2 | sequence background | MIKCc-type MADS-box gene proposed as a putative MpANT target via APB-binding-site/co-expression inference. |
| Mp8g17460 | MpPYL1 | sequence background | ABA-receptor gene most strongly co-expressed with MpANT and proposed as a putative target. |
| — | MpRDR5 missing | citation background | Co-expressed with MpANT in the young sporophyte region. |
| — | MpRDR6-2 missing | citation background | Co-expressed with MpANT in the young sporophyte region. |
| Mp3g19510 | MpSHI | citation background | SHI-related gene noted in the MpANT co-expression cluster. |
| Mp1g10030 | MpSPL2 | sequence background | SPL-like transcription factor proposed as a putative MpANT target based on co-expression and APB-binding-site inference. |
| Mp1g11660 | MpWOX | citation background | Cited from Fu et al. as a downstream gene by which MpANT promotes meristem maintenance. |