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The conserved machinery of bipolar prospindle formation controls acentrosomal spindle orientation in land plants.

Sasaki T, Ishizaki K, Motose H, Oda Y. · 2025 · Current biology : CB   research

doi:10.1016/j.cub.2025.08.038   PMID:40945508

Linked genes (7)  2 core 5 peripheral

Gene ID Name Evidence / Role Function (this paper)
MpCORD missing experimental subject The single M. polymorpha CORD gene; Mpcord cells were used to dissect bipolar prospindle formation, showing a conserved machinery that controls acentrosomal spindle orientation in land plants.
MpKTN missing experimental subject Microtubule-severing katanin; loss of MpKTN leads to failure in preprophase-band (PPB) formation and fragmentation of the polar microtubule arrays.
MpAUR missing experimental tool Aurora kinase imaged via pMpAUR:mCitrine-MpAUR as a spindle marker in wild-type and Mpcord cells.
MpGIP1 missing experimental tool GCP3-interacting protein imaged via pMpGIP1:MpGIP1-mCitrine as a prospindle/MTOC marker.
MpLTI6Ba missing experimental tool Plasma-membrane marker (mScarlet-I-MpLTI6Ba) used to outline cells during imaging.
MpTPX2 missing experimental tool TPX2 spindle assembly factor imaged via pMpTPX2:MpTPX2-mCitrine during prospindle formation.
Mp2g09390 MpTUB2 experimental tool beta-tubulin used as a microtubule marker (mCitrine-MpTUB2) to image mitotic microtubule structures.